LUNDGREN, DONALD G. Microbiological problems in strip mine areas: Relationship to the metabolism of Thiobacillus ferrooxidans. Ohio J. Sci. 75(6): 280, 1975. The role of the iron oxidizing bacteria in contributing to acid mine drainage is considered relative to the metabolism of the organism. To study acid production an in vitro system was developed using isolated cell envelopes from Thiobacill...

The rusticyanin gene from the acidophilic chemolithotroph Thiobacillus ferrooxidans has been cloned and sequenced. A central portion of the gene was identified by PCR reactions utilising primers optimised for codon bias followed by nested PCR with degenerate primers. The 5' and 3' ends of the rusticyanin gene were then cloned using degenerate primers to each end and anchor sequences to the know...

The growth of Thiobacillus ferrooxidans on sulphur is known to proceed through the attachment of cells to the sulphur particles. Experiments, However, show that the cells in the liquid phase, which are not attached to the sulphur particles, also grow. It has been shown through the use of a two-compartment membrane reactor that this increase is partially due to the release of ions, corresponding...