This specific phosphatase is a 5-nucleotidase; it differs from the alkaline phosphatase in its range of action and in its optimal pH. Whereas alkaline phosphatase, which is non-specific, hydrolyses a great variety of phosphoric esters, the 5-nucleotidase hydrolyses only nucleotides with the phosphate group attached to the fifth carbon of the pentose, that is, muscle adenylic acid (adenosine-5-m...

Acid phosphatase activity increases above normal in the liver of whole body irradiated animals. It is considered to be one of the adverse radiation effects. Increase in the acid phosphatase activity was dose dependent. Centella asiatica extract, which increased the survival of gamma irradiated Swiss albino mouse when given prior to irradiation was tested to observe changes in the acid phosphata...

Increased acid phosphatase activity in the serum and tissues of patients with Gaucher's disease has now been recognized for two decades, but as yet no relation has been established between the enzyme and the etiology and progress of the disease. Here, we review results obtained by various investigators, ranging from a consideration of the methods used for the evaluation of serum acid phosphatas...

UNLABELLED The presence of semen in vaginal fluid, as identified by an acid phosphatase spot test, does not influence vaginal proinflammatory cytokine concentrations. OBJECTIVE Determine whether semen, as detected by acid phosphatase, influences vaginal cytokines or secretory leukocyte protease inhibitor concentrations. METHODS 138 pregnant women had vaginal fluid collected for Gram stain, ...

The major secreted isoenzyme of human prostatic acid phosphatase (PAcP) (EC 3.1.3.2), which catalyses p-nitrophenyl phosphate (PNPP) hydrolysis at acid pH values, was found to have phosphotyrosyl protein phosphatase activity since it dephosphorylated three different phosphotyrosine-containing protein substrates. Several lines of evidence are presented to show that the phosphotyrosyl phosphatase...

Lysosomal enzymes acid phosphatase show increase in diabetes mellitus and this is closely related to diabetic metabolic alterations. This was a crosssectional study conducted on 50 diabetic subjects (25 patients with complications and 25 patients without complications) in RIMS hospital during the period from September 2010 to August 2012. Twenty five age and sex matched healthy individual were ...

An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange chromatography on DEAE cellulose. The acid phosphatase gave a single band on SDS-polyacrylamide slab gel electrophoresis. The molecular weight of the aci...

Lysosomal acid phosphatase levels are reduced in murine macrophages by virulent strains of Nocardia asteroides. At the same time, other lysosomal enzymes either remain unchanged or increase in activity, indicating that acid phosphatase is not lost because of degranulation or membrane leakage. This study shows that acid phosphatase was utilized as a sole carbon source by Nocardia asteroides and ...

”he acid phosphatase activity that is elevated 3to 5fold in the spleen in Gaucher’s disease can be resolved into two principal isoenzymes by chromatography on sulphopropyl-Sephadex: one tartrate-sensitive component does not bind to the resin whereas the second acid phosphatase, which is tartrate-resistant, binds to the column and can be eluted by a buffer containing 0.4 M sodium chloride. We ha...

In this study, we isolated cDNA encoding lysophosphatidic acid (LPA) phosphatase (LPAP). The amino acid sequence deduced from the cDNA encoding LPAP had 421 residues including a putative signal peptide and was homologous to acid phosphatase, especially at the active site. Human LPAP had 28.5% amino acid identity to human prostatic acid phosphatase. Northern blot analysis showed a ubiquitous exp...