1. The hydrolysis of monolayers of phosphatidyl[Me-(14)C]choline at the air/water interface by phospholipase D (phosphatidylcholine phosphatidohydrolase) was investigated by a surface-radioactivity technique by using a flow counter. 2. Phosphatidylcholine of high specific radioactivity was prepared biosynthetically in good yield from [Me-(14)C]choline by using Saccharomyces cerevisiae. 3. At in...

Solid acid catalysts, which have favorable characteristics such as efficient activity, high selectivity, long catalyst life and ease in recovery and reuse, have great potential for efficiently transforming lignocellulosic biomass into biofuels and can replace many conventional liquid acids for hydrolysis and pretreatment. This work briefly introduces conventional biomass pretreatment and hydrol...

Described here is the sonolytic hydrolysis of peptides achieved by treatment of aqueous solution to which the radical scavenger 1,4-benzenedithiol (1,4-BDT), which has hydrogen donating ability, has been added. Mass spectrometric analysis of the products of sonolytic hydrolysis gave information about amino acid sequence of the peptides without any byproducts. The additive 1,4-BDT improves the s...

Xanthine oxidase (XO), the main enzyme responsible for formation of uric acid, is abundant on milk fat globule membrane (MFGM), which might be associated with increasing risk hyperuricemia from high-fat compared to low-fat dairy product. In our study, we hypothesized that XO activity could maintained after hydrolysis under conditions simulating digestion and produce acid either in blood or inte...

Abstract Background Methods to produce XOS have been intensively investigated, including enzymatic hydrolysis, steam explosion, and acid hydrolysis. Acid hydrolysis is currently the most widely used method due its advantages of fewer processing steps, stronger raw material adaptability, higher yield, better reproducibility. Especially, organic acids such as acetic acid, formic xylonic work comp...

The aim of this pharmacokinetic evaluation was to show the effect of the extra methyl group in simvastatin on esterase hydrolysis between lovastatin and simvastatin in male and female volunteers. This study was based on the plasma concentration-time curves and the pharmacokinetics of lovastatin and simvastatin with its respective active metabolite statin-beta-hydroxy acid obtained from two diff...

Jatropha hulls were successfully for the first time used as raw materials for the production of 2,3-butanediol via dilute sulfuric acid hydrolysis and fermentation with Klebsiella oxytoca. Two-step hydrolysis was used to effectively hydrolyze the hulls at 150°C after pretreatment. In the first-step, hemicellulose was hydrolyzed under mild conditions (0.5h, 1.5% acid) to avoid secondary decompos...

Minerals can readily bind to phytic acid and thus have the potential to form mineral±phytate complexes that may be resistant to hydrolysis by phytase activity of animal, plant and microbial origin. In simple solution, at pH 7.0, mineral concentrations from 0.053 mM for Zn up to 4.87 mM for Mg caused a 50% inhibition of phytate-P hydrolysis by microbial phytase. The rank order of mineral potency...

By DFT calculation, we found that acid-catalyzed carboxylic acid esterification and ester hydrolysis are brief two-step reactions. First, the carboxylic acid hydroxyl-oxygen or ester alkyl-oxygen is protonated, which generates a highly active acylium ion. The protonation requires an activation energy (Ea) of 4-10 kcal mol(-1), and is the rate-controlling step of the esterification or hydrolysis...

The behaviour of different cellulosic fibres during acid hydrolysis has been investigated and the levelling-off degree of polymerisation (LODP) has been determined. The study included a bleached kraft pulp (both never-dried and once-dried) and two dissolving pulps (once-dried). Additionally, cotton cellulose from new cotton sheets and sheets discarded after long-time use was studied. Experiment...