The development of functional foods and their safety assessment is required for well-being the human health. In present study, we have isolated identified a potential probiotic bacterium from fermented garlic pickle that exhibits an improvement in health status upon consumption. order to assess bacterium, vitro tests were performed, including tolerance acid, pancreatin,bile, adhesion ability ga...

AIMS To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS Spores and vegetative cells were challenged in vitro miming the injury of gastrointestinal transit: pH variations, exposure to conjugated and free bile salts, micr...

Abstract Purpose Streptococcus alactolyticus strain FGM is used to ferment Astragalus membranaceus develop a novel feed additive for animals in China. This study aimed at characterizing the safety and potential probiotic features of vitro. Methods The genome S. was sequenced genomic silico studies. It evaluated morphology, antibiotic susceptibility, hemolytic activity, acid tolerance, bile salt...

The purpose of this study was to evaluate the probiotic Bifidobacterium animalis subsp. lactis BB-12 with regard to its resistance to simulated gastrointestinal stress. The tested strain was able to survive both in the presence of 0.3% bile salts for 5h and in the acidified MRS broth with pH 2.0 for 2h, the rate of viability being by 78% and 76% respectively. The presence of pepsin with a view ...

Transcriptional coregulators are important components of nuclear receptor (NR) signaling machinery and provide additional mechanisms for modulation of NR activity. Expression of a mutated nuclear corepressor 1 (NCoR1) that lacks 2 NR interacting domains (NCoRΔID) in the liver leads to elevated expression of genes regulated by thyroid hormone receptor (TR) and liver X receptor (LXR), both of whi...

BACKGROUND/AIMS Primary biliary cirrhosis (PBC), an autoimmune disease of the liver, is marked by slow progressive destruction of bile ducts. These patients with PBC often undergo orthotopic liver transplantation (OLT). Ischemic bile duct lesion (IBDL) is a major source of morbidity and even mortality after OLT. Cirrhosis of the liver has a higher tolerance to ischemia than a normal liver, but ...

Recent guidelines indicate that oral probiotics, living microorganisms able to confer a health benefit on the host, should be safe for human consumption, when administered in a sufficient amount, and resist acid and bile to exert their beneficial effects (e.g., metabolic, immunomodulatory, anti-inflammatory, competitive). This study evaluated quantitative and qualitative aspects and the viabili...

G protein-coupled bile acid receptor 1 (TGR5) serves a key function in regulating glycometabolism. TGR5 is highly expressed in the mitochondria of brown adipose tissue (BAT) and downregulates adenosine triphosphate synthesis via the bile acid-TGR5-cyclic adenosine monophosphate-2-iodothyronine deiodinase (D2)-triiodothyronine-uncoupling protein pathway, thus regulating energy homeostasis and re...