The primary alcohol/aldehyde dehydrogenase (coded by the aad gene) is responsible for butanol formation in Clostridium acetobutylicum. We complemented the non-sporulating, non-solvent-producing C. acetobutylicum M5 strain (which has lost the pSOL1 megaplasmid containing aad and the acetone-formation genes) with aad expressed from the phosphotransbutyrylase promoter and restored butanol producti...

lead is a common toxic agent which impairs various organs functions of the body. the basis of this toxicity is believed to be the negative influence on enzymatic system of cells. in the past few years, increasing consideration has been given to interactions occurring between lead and some essential metals including se and zn. based on the finding that some trace elements could reverse the toxic...

enzymatically prepared by removing acyl moieties from the cyclohexane ring of LSNs, and its thrombopoietic effects. Furthermorewealso report on the structureactivity relationship of its derivatives. A mixture of LSNswas treated with porcine liver esterase (PLE-A) in acetone-phosphate buffer (pH = 6.7) at 37°C, after filtering off the enzyme, purification by Cosmosil column afforded LSN-H (Id) a...

Europium (III) complex with anthracene-9-carboxylic acid (9-AA) has been synthesized and characterized by elemental analysis, FT-IR, and TG-DTG techniques. The results indicated that the composition of this complex is Eu (9-AA)(3). The luminescence properties of the complex in different solvents and at different pH values have been investigated. The results show that the complex exhibits more e...

As a follow-up to our effort establish reliable thermodynamic data for amino acids, the heat capacity and phase behavior are reported N-acetyl glycine amide (CAS RN: 2620-63-5), N-acetyl-L-alanine 15962-47-7), N-acetyl-L-valine 37933-88-3), N-acetyl-L-isoleucine 56711-06-9), N-acetyl-L-leucine 28529-34-2). Prior measurement, thermogravimetric analysis X-ray powder diffraction were performed det...

In this study, solid phase micro-extraction (SPME) was optimized to improve the sensitivity and accuracy in formaldehyde determination for plywood panels. Further work has been carried out to compare the newly developed technique with existing method which reacts formaldehyde collected in desiccators with acetyl acetone reagent (DC-AA). In SPME, formaldehyde was first derivatized with O-(2,3,4,...

A type of iron-bound protein was isolated from Clostridium botulinum by a modification of the method used for isolating ferredoxin from C. pasteurianum. This method involved acetone and diethylaminoethyl cellulose treatments followed by ammonium sulfate fractionation. The protein exhibited maximal absorption in the ultraviolet region near 260 mmu. Portions of the isolated iron protein were sepa...

130 species, mostly trees, confined to the warm humid tropics of the world. Some species are used in traditional medicine for their antimicrobial, analgesic, gastroprotective activities and many of them are acknowledged for the presence of pyranocoumarins which act as potent inhibitors of HIV-1 replication and cytopathicity. Besides, the genus is considered as a rich source of phenolic compound...

Assay System-The assay system, previously described (1, 6, 7), is based on the conversion of acetyl CoA to citrate in the presence of malic dehydrogenase and condensing enzyme. The oxalacetate necessary for the condensation reaction is generated by the oxidation of malate in the presence of DPN, and the rate of DPN reduction is proportional to the concentration of AAE present, within the limits...