We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (COA) carboxylase (ACCase) of Arabidopsis tbaliana (CAC7). l h e 3' end of the CAC7 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was expressed in Escbericbia coli as a glutathione-S-transferase (CST) fusion pr...

Littleseed canarygrass (Phalaris minor Retz.) is one of the most common and troublesome weeds infesting wheat crop in India. Repeated use during the last two decades of the ACCase-inhibiting herbicide (clodinafop) to control this weed has resulted in the occurrence of resistance. Fifty-three P. minor populations were collected from wheat fields in Haryana and Punjab states of India. The dose-re...

The United States is the largest producer of maize in the world. Maize-soybean is the most prominent crop rotation in the Midwestern United States. Glyphosate-resistant volunteer maize is a problem weed not only in soybean, but also in continuous maize or sugarbeet rotations. Storm damage, harvesting problems, poor stalk quality, and insect damage, among other factors, can lead to kernel and ea...

Natural accessions of Arabidopsis (Arabidopsis thaliana) differ in their ability to tolerate a loss of chloroplast translation. These differences can be attributed in part to variation in a duplicated nuclear gene (ACC2) that targets homomeric acetyl-coenzyme A carboxylase (ACCase) to plastids. This functional redundancy allows limited fatty acid biosynthesis to occur in the absence of heterome...

We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was expressed in Escherichia coli as a glutathione-S-transferase (GST) fusion prot...

Acetyl-CoA carboxylase (ACCase) catalyses the carboxylation of acetyl-CoA, forming malonyl-CoA, which is used in the plastid for fatty acid synthesis and in the cytosol in various biosynthetic pathways including fatty acid elongation. In Arabidopsis thaliana, ACC1 and ACC2, two genes located in a tandem repeat within a 25-kbp genomic region near the centromere of chromosome 1, encode two multif...

Black-grass is a common grass weed, widely spread in Northern Europe and also in Belgium. For ages, it has been an increasing problem in industrial crops, especially winter cereals. The first case of resistance in Belgium was reported in 1996 by Robert Bulcke (Eelen et al., 1996). Yet the resistance mechanism was not specified. Since then, no more information was published about the evolution B...

Echinochloa spp. is one of the most invasive weeds in rice fields worldwide. Acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibiting herbicides are two widely used herbicides. However, overuse has led to resistance evolution penoxsulam (ALS-inhibitor) cyhalofop-methyl (ACCase-inhibitor). In this work, 137 different populations were collected Extremadura (western Spain) where ...

Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to any known protein. Expression of accB and accE in Escherichia coli and subsequen...