Open Access Oral presentation 2.2 A phase II trial with canakinumab, a new IL-1beta blocking monoclonal antibody (ACZ885), to evaluate preliminary dosing, safety and efficacy profile in children with systemic Juvenile Idiopathic arthritis (sJIA) N Ruperto*1, P Quartier2, N Wulffraat3, P Woo4, A Loy1, R Mouy2, B BaderMeunier2, B Prakken3, E Noseda5, C Rordorf5 and Martini for the Paediatric Rheu...

A 30-year-old G2P1 female is 12 weeks pregnant and her red blood cells (RBCs) type as A1 with a negative antibody screen. The transfusion medicine service learns that the patient typed as A– during her first pregnancy and she received Rh immune globulin (RhIG). How would you proceed to clarify the patient’s D type and to evaluate the need for prophylaxis to prevent hemolytic disease of the fetu...

Antibodies to Streptococcus pneumoniae 6B capsular polysaccharide (PS) induced with a 23-valent PS vaccine among 25 adults were examined. The magnitude of antibody responses among different subjects was highly correlated with the amount of anti-6B antibodies expressing IgG (r = 0.98) and lambda (r = 0.93) isotypes. Most individuals produced one or two dominant IgG antibody clones as identified ...

Short cDNA fragments covering the entire coding region for apolipoprotein (apo) B have been cloned in the pING expression vector. A monoclonal antibody specific for the Ag(d) epitope on apo B has been used, together with the expressed apo B peptides, to locate this epitope to a stretch of 26 amino acids. Sequencing of this region from several genomic DNAs of known Ag(a1/d) genotype showed a sin...

Patients with antiphospholipid syndrome are characterized by the association of thrombosis or pregnancy morbidity and the presence of antiphospholipid autoantibodies. Particularly, anti-beta(2)-glycoprotein (beta(2) GPI) autoantibodies correlate with thrombosis, suggesting an antibody-induced gain of prothrombotic function and/or an antibody-induced loss of antithrombotic function of beta(2) GP...

A cardinal feature of generalised nodal osteoarthritis is the loss of articular cartilage. To determine if autoimmunity to these cartilage collagens occurred, serum antibodies to native and denatured type II collagen were measured by enzyme linked immunosorbent assay (ELISA) in 96 patients (90 women, six men, aged 47-91 years) with generalised nodal osteoarthritis. Generalised nodal osteoarthri...

Binding of the adhesive glycoprotein, von Willebrand factor (vWf), to the platelet membrane glycoprotein (GP) Ib-IX-V complex initiates platelet adhesion and aggregation at high shear stress in hemostasis and thrombosis. In this study, the GP Ib-IX-V binding site within the vWf A1 domain was analyzed using a panel of murine monoclonal antibodies raised against a 39/34-kd vWf fragment (Leu-480/V...

Plasma prekallikrein, a zymogen of the contact phase system, circulates in plasma as heterodimeric complex with H-kininogen. The binding is mediated by the prekallikrein heavy chain consisting of four apple domains, A1 to A4, to which H-kininogen binds with high specificity and affinity (K(D) = 1.2 x 10(-8) M). Previous work had demonstrated that a discontinuous kininogen-binding site is formed...

Various osteogenic tumours and bone producing tumour-like lesions of bone were examined for S-100 protein using the immunostaining methods. The positive reaction for S-100 protein of some bone cells was detected not only in osteoblastic osteosarcomas and osteoblastomas, but also in osteoplastic reactive lesions, fibrous dysplasia, Paget's disease and in the tissue of the bony callus. The positi...

The concentrations of serum amyloid A (SAA) and a1•acid glycoprotein (AAG) in the serum of 11 healthy cats subjected to oophorohysterectomy (5 females) and gastrotomy (3 males, 3 females) were measured by turbidimetric immunoassay (TIA) for human SAA (human SAA-TIA) and single radial immunodiffusion (SRID) kits for feline AAG, respectively. Six healthy cats (3 males, 3 females) were inoculated ...