κ-casein is a glycosilated protein belonging to a family of phosphoproteins (αs1,β, αs2,κ) that represents the major protein component in mammalian milk. κ-casein plays an essential role in the case of micelle stabilization, determining the size and the specific biological function. In the present study, we report the characterization of the partial sequence of κ-casein promoter region in the I...

κ-casein is a glycosilated protein belonging to a family of phosphoproteins (αs1,β, αs2,κ) that represents the major protein component in mammalian milk. κ-casein plays an essential role in the case of micelle stabilization, determining the size and the specific biological function. In the present study, we report the characterization of the partial sequence of κ-casein promoter region in the I...

toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. toxoplasma gondii-derived heat shock protein 70 (hsp70) may play an important role in the virulence of toxoplasma gondii (t. gondii). in the present study, t. gondii hsp70 was amplified by polymerase chain reaction (pcr) from the dna of the t. gondiitachyzoite rh strain through the use o...

Little information is available regarding genetic variation in wild wheat relatives from Iran. In this study, genetic diversity of 36 populations of wild einkorn wheat, Triticum boeoticum, was studied using amplified fragment length polymorphism (AFLP) primer combinations. Seventeen AFLP primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 (44%) ...

Self-incompatibility has been studied by using controlled pollination, pollen tube growth and PCR methods in the Iranian almond ‘Mamaei.’. Pollen tube growth and fruit set following self and cross-pollination treatments were evaluated. The percentage of initial and final fruit set was determined for each treatment at 30 and 60 days after controlled pollination. Pollen germination and pollen ...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study the polymorphism of the cast gene ofkhalkhali goat in azerbaijan province in iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (pcr-rflp). genomic dna was extracted from whole blood samples collected from 200 kh...

A novel, accurate, rapid and modestly labor-intensive method has been developed to quantitate specific mRNA species by reverse transcription-polymerase chain reaction (RT-PCR). This strategy combines the high degree of specificity of competitive PCR with the sensitivity of laser-induced fluorescence capillary electrophoresis (LIF-CE). The specific target mRNA and a mimic DNA fragment, used as a...

A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...