The mechanism of inhibition of gastric peroxidase (GPO) activity by mercaptomethylimidazole (MMI), an inducer of gastric acid secretion, has been investigated. Incubation of purified GPO with MMI in the presence of H2O2 results in irreversible inactivation of the enzyme. No significant inactivation occurs in the absence of H2O2 or MMI, suggesting the involvement of peroxidase-catalysed oxidized...

Murine norovirus (strain MNV-1), a propagable norovirus, was evaluated for susceptibility to high-pressure processing. Experiments with virus stocks in Dulbecco's modified Eagle medium demonstrated that at room temperature (20 degrees C) the virus was inactivated over a pressure range of 350 to 450 MPa, with a 5-min, 450-MPa treatment being sufficient to inactivate 6.85 log(10) PFU of MNV-1. Th...

Incorporation of black soldier fly larvae (BSFL) in fecal sludge management shows promise as a resource recovery strategy. BSFL efficiently convert organic waste into valuable lipids and protein, which can be further processed commercial products. Ensuring the microbial safety waste-derived products is critical to success resource-oriented sanitation requires development effective treatment. Th...

We demonstrate a diamond Raman laser intracavity-pumped by a red semiconductor disk laser (~675 nm) for laser emission at around 740 nm. Output power up to 82 mW of the Stokes-shifted field was achieved, limited by the available pump power, with an output coupling of 1.5%. We also report wavelength tuning of the diamond Raman laser over 736 - 750 nm.

The object of the present work was to design, construct and evaluate a cylindrical tri-axial charger for charging of submicron aerosol particles by unipolar ions. The corona discharge characteristics, the intrinsic and extrinsic particle charging efficiencies, and the losses of aerosol particles were experimentally evaluated for particle diameters in the range between 50 nm and 500 nm under dif...

Survival and growth of Listeria monocytogenes L56 (IMR isolate) was studied in trypticase soy broth (TSB) grown at 37oC before being subjected to three selected sublethal stress temperatures (55oC, 28oC and 4oC). The morphological changes and motility as affected by temperature stresses were determined using Scanning Electron Microscopy (SEM) and motility media, respectively. For this purpose, ...

The effect of Mg2+ on the thermal inactivation and unfolding of rabbit muscle creatine kinase has been studied for various temperatures and Mg2+ concentrations. Increasing the Mg2+ concentration in the denatured system significantly enhanced the inactivation and unfolding of creatine kinase during thermal denaturation. The analysis of the kinetic course of substrate reaction during thermal inac...

The mechanism of suicidal inactivation of lactoperoxidase (LPO) by mercaptomethylimidazole (MMI) has been studied. Analogue studies indicate a specific requirement for the thiol group of MMI for inactivation of LPO in the presence of H2O2. MMI is oxidized via one-electron transfer by LPO compound II as demonstrated by a spectral shift from 430 to 412 nm through an isosbestic point at 421 nm. A ...

Efficient generation of widely tunable picosecond pulses from the visible to near-infrared is demonstrated by optical parametric generation and amplification in BiB(3)O(6). Pumped by the second harmonic of an amplified mode-locked Nd:YAG laser at 532 nm, also generated in BiB(3)O(6), a signal and idler tuning range of 740-1893 nm has been achieved with angle tuning under type I (o?e+e) phase-ma...