Ubiquitination occurs at synapses, yet its role remains unclear. Previous studies demonstrated that the RPM-1 ubiquitin ligase organizes presynaptic boutons at neuromuscular junctions in C. elegans motorneurons. Here we find that RPM-1 has a novel postsynaptic role in interneurons, where it regulates the trafficking of the AMPA-type glutamate receptor GLR-1 from synapses into endosomes. Mutatio...

Positioning rRNA within the ribosome remains a challenging problem. Such positioning is critical to understanding ribosome function, as various rRNA regions interact to form suitable binding sites for ligands, such as tRNA and mRNA. We have used phenanthroline, a chemical nuclease, as a proximity probe, to help elucidate the regions of rRNA that are near neighbors of the stem-loop structure cen...

Journal of Comparative NeurologyVolume 530, Issue 6 COVER IMAGEFree Access Cover Image, Volume First published: 10 February 2022 https://doi.org/10.1002/cne.25309AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text full-text accessPlease review our Terms and Conditions Use check box below share version article.I have read accept the...

1 University of California, Davis. Department of Biomedical Engineering. Genome and Biomedical Sciences Facility, 451 E. Health Sciences Dr., Davis, California, 95616-5294. 530-752-1430 (U. S., A. F. H. L., M. R. S.) 530-754-2193 (C. E. G.), 530-752-0299 (S. I. S.). Fax 530-754-5739 2 ICOS Corporation, 22021 20 Ave. SE, Bothell, Washington 98021. 3 Current Address: The Scripps Research Institut...

A nine-base DNA oligomer [d(GTATCTAAT)] was used to probe the accessibility and function of bases in the region 787-795 of Escherichia coli 16S rRNA. Hybridization of the cDNA [d(GTATCTAAT)] to 16S rRNA in situ was carried out by binding the probe to intact 30S ribosomal subunits. Nitrocellulose filter binding showed that cDNA hybridization saturated with increasing probe concentration, suggest...

Abstract is not required for Clinical Imagesis not required for Clinical Images (This page in not part of the published article.) International Journal of Case Reports and Images, Vol. 5 No. 11, November 2014. ISSN – [0976-3198] Int J Case Rep Images 2014;5(11):795–797. www.ijcasereportsandimages.com Flynn et al. 795 clinical images OPen access Early bilateral hippocampal lesions in transient g...

RPM is a package management system which provides a uniform, automated way for users to install, upgrade, and uninstall programs. Because RPM is the default software distribution format for many operating systems (particularly GNU/Linux), users may find it useful to manage their library of TEXrelated packages using RPM. This article explains how to produce RPM files for TEX software, either for...

Introduction 528 Method evaluation 528 Sample collection 530 Isolation methods 530 Preenrichment 530 Enrichment 531 Agar plates 531 Methods for bacterial counts 532 Pathogenic bacteria 533 Campylobacter 533 Clostridium perfringens 536 Listeria 536 Salmonella 537 Spoilage microorganisms 538 Mesotrophic bacteria 538 Psychrotrophic bacteria 539 Yeasts and molds 539 Application of molecular techniq...

Changes in axon outgrowth patterns are often associated with synaptogenesis. Members of the conserved Pam/Highwire/RPM-1 protein family have essential functions in presynaptic differentiation. Here, we show that Caenorhabditis elegans RPM-1 negatively regulates axon outgrowth mediated by the guidance receptors SAX-3/robo and UNC-5/UNC5. Loss-of-function rpm-1 mutations cause a failure to termin...