As part of a large, ongoing study of invasive infections in pediatric patients in Bamako, Mali, 106 cases of invasive pneumococcal disease were identified from June 2002 to July 2003 (J. D. Campbell et al., Pediatr. Infect. Dis. J. 23:642-649, 2004). Of the 12 serotypes present, the majority of isolates were not contained in PCV7 (the 7-valent pneumococcal conjugate vaccine), including 1 isolat...

A listeriosis outbreak in the United States implicated contaminated ice cream produced by one company, which operated 3 facilities. We performed single nucleotide polymorphism (SNP)-based whole genome sequencing (WGS) analysis on Listeria monocytogenes from food, environmental and clinical sources, identifying two clusters and a single branch, belonging to PCR serogroup IIb and genetic lineage ...

ISSN: 2319-7706 Volume 4 Number 5 (2015) pp. 172-190 http://www.ijcmas.com Neonatal sepsis is a leading cause of neonatal mortality in developing countries. Identification of the etiological agents of neonatal sepsis is essential for effective treatment. Out of 106 microbial isolates recovered from blood cultures of late onset neonatal sepsis (LONS) patients, only 70 (66 %) isolates were Gram-p...

OBJECTIVE To evaluate enterotoxin production, enterotoxin gene distribution, and genetic diversity of Staphylococcus aureus in milk obtained from cows with subclinical mastitis. SAMPLE Milk samples obtained from 350 cows (1,354 mammary glands) on 11 Wisconsin dairy farms. PROCEDURES Of 252 S aureus isolates obtained from 146 cows, 83 isolates (from 66 cows with subclinical mastitis) were co...

OBJECTIVE To establish the relatedness of methicillin-resistant Staphylococcus aureus (MRSA) isolates in the Maternity Hospital, Kuwait. MATERIALS AND METHODS A total of 22 MRSA were isolated from 20 neonates and 1 mother in the Special Care Unit, Maternity Hospital, Kuwait. They were characterized using antibiogram, pulsed-field gel electrophoresis (PFGE), SCCmec typing, spa typing and multi...

Antifungal activities of two essential oils (EOs), derived from the Lavandula angustifolia L. and Thymbra spicata plant leaves were tested in current study against isolates (M1-5 M3-5) Botrytis cinerea Potato Dextrose Agar (PDA). These studies performed vitro a further vivo test with vapor contact application EOs was strawberry fruits to confirm antifungal postharvest storage. In conducted four...

OBJECTIVE To determine genetic relatedness of clone Colombia(5) ST289 with invasive Streptococcus pneumoniae serotype 5 isolates recovered in nine Latin American countries. METHODS Forty-four invasive S. pneumoniae serotype 5 isolates recovered from children under 5 years of age in Bolivia, Chile, Dominican Republic, Ecuador, Nicaragua, Panama, Paraguay, Peru, and Venezuela were studied. Puls...

A prolonged incubation time is generally recommended for diagnosing periprosthetic joint infections (PJI). However, in literature, no distinction made between acute and chronic infections. All patients with a PJI that underwent surgical debridement November 2015 February 2019 or without revision of the prosthesis were retrospectively evaluated. Synovial fluid, 5 intraoperative tissue samples, s...

DNA fingerprinting of Candida dubliniensis isolates using the species-specific probe Cd25 previously showed that this species consists of two distinct groups, termed Cd25 group I and Cd25 group II. The present study investigated the population structure of 30 C. dubliniensis oral isolates from Saudi Arabia and Egypt using Cd25 fingerprinting and rRNA gene internal transcribed spacer region-base...