Plant viruses are generally considered incapable of infecting vertebrates. Accordingly, they are not considered harmful for humans. However, a few studies questioned the certainty of this paradigm. Tobacco mosaic virus (TMV) RNA has been detected in human samples and TMV RNA translation has been described in animal cells. We sought to determine if TMV is detectable, persists, and remains viable...

BACKGROUND To assess the effect of rostral anterior cingulate cortex (rACC) administration with ifenprodil (NR2B receptor blocker) on bone cancer pain (BCP)-related aversion sentiment using the conditioned place avoidance experiments in rats. METHODS A total of 30 male Wistar rats without place preference were randomly assigned to three groups: control group (Group C, n = 10), BCP group (Grou...

While some strains of ECHO 9 virus were found to be completely incapable of multiplying in newborn mice or even of being adsorbed by their tissues (e.g., the prototype Hill strain), other naturally occurring strains readily multiplied even after inoculation of as little as 3 TCD(50) of virus. With the multiplying strains, the infection remained clinically inapparent except after inoculation of ...

Portland-composite cements (PCC) were prepared with partial substitution of 5 to 25% class C fly ash, obtained from East Java steam power plant. Properties the composite was evaluated, through setting time (initial and final) compressive strength (3, 7, 28 days) compared control PCC. Setting is retarded in higher ash content. The retardation highest PCC ash. Lower after 3 7 days curing for 5-25...

AIM To use a confocal microscope to characterise the treated and untreated courses of fungal keratitis. METHODS In the first experiment, Aspergillus fumigatus stromal keratitis was produced in both eyes of seven New Zealand white rabbits. In the second experiment, keratitis was induced in right eyes of 20 rabbits. Group 1 rabbits were treated with topical fluconazole, group 2 rabbits received...

A cell culture infection test was developed for the isolation ofrabies virus from field cases submitted for rabies diagnosis. The procedure involved the addition of a suspension of suspect brain tissue to a suspension of murine neuroblastoma cells in 96-well microtiter plates. The cultures were then incubated at 35-36° C for four days at which time they were fixed, stained with a fluorescein-la...

fire blight, caused by the bacterium erwinia amylovora, is one of the most destructive diseases of plants in the rosaceae subfamily pomoideae. the objective of this study was to determine the level of resistance of 30 pear cultivars and describe the biochemical changes induced in some resistant and susceptible cultivars. the level of resistance was determined by the length of the fire blight le...

This study was conducted in acompletely randomized block design with three replications using three concentrations of 107, 105, and 103cfu/ml of Azotobacter isolated from the rhizosphere of Karun varieties of barley (Az. salentris) and standard Az.chrococom along with control (zero concentration of bacteria). The aim of this study was to study the reactions of barley plantin response to inocula...

The indirect fluorescent antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in...