A new radical mechanism of nucleotide polymerization is found. The finding is based on the Car-Parrinello molecular dynamics computations at 310 K with an additional spin-spin coupling term for 31P and 1H atoms and a radical pair spin term included. The mechanism is initiated by a creation of a high-energy spin-separated Mg-ATP complex in a triplet state in which the Mg prefers an uncommon chel...

We compared saline (S) and sodium dodecyl sulphate (SDS) extracts from Taenia solium (homologous species - HO) and Taenia crassiceps (heterologous species - HE) metacestodes in order to detect IgG by ELISA and immunoblot assay (IBA) in cerebrospinal fluid (CSF) for the diagnosis of human neurocysticercosis (NC). CSF samples were obtained from 93 patients. Of these, 40 had NC, five had a diagnos...

Hyung-Ho Kim, Sang-Uk Han1, Min-Chan Kim2, Woo Jin Hyung3, Wook Kim4, Hyuk-Joon Lee5, Seung Wan Ryu6, GyuSeok Cho7, Chan Young Kim8, Han-Kwang Yang5, Do Joong Park, Kyo Young Song9, Sang IL Lee10, Seong Yeob Ryu11, Joo Ho Lee12; Korean Laparoscopic Gastrointestinal Surgery Study (KLASS) Group Department of Surgery, Seoul National University Bundang Hospital, Seoul National University College of...

Rational combination of pyridazine, hydroxo and carboxylate bridging ligands led to the assembly of three types of mixed-ligand polynuclear Cu(II) clusters (A: [Cu2(μ-OH)(μ-pdz)(μ-COO)]; B: [Cu4(μ3-OH)2(μ-pdz)2]; C: [Cu5(μ-OH)2(μ-pdz)4(μ-COO)2(μ-H2O)2]) and their integration into 3D framework structures. Mixed-ligand complexes [Cu2(μ-OH){TMA}(L)(H2O)] (1), [Cu4(μ3-OH)2{ATC}2(L)2(H2O)2]·H2O (2) ...

BACKGROUND Abundance of commensals constituting the intestinal microbiota (IM) affects the immune system and predisposes to a variety of diseases, including intestinal infections, cancer, inflammatory and metabolic disorders. Housing conditions determine the IM and can hence influence the immune system. We analyzed how both variables affect the IM of four immune-compromized mouse lines kept und...

☆ This work was supported by a grant of The Advancing Safe Transportation throughout the Lifespa G-0058) to MB, a fellowship from the Swiss Nationa (PA001-117473) to SMJ, and a grant from the Office o (N00014-09-0213). Study 2 was conducted as a part with the guidance of the other co-authors and wit University of Bern and the National Taiwan Normal U wish to thank Chao-Yi Ho and Philip Cheng fo...

The purpose of this study is to compare three-dimensional conformal radiotherapy (3D-CRT) plans in a setting of salvage radiotherapy after radical prostatectomy (RP) and to simulate whether dose escalation is possible with the most adequate 3D-CRT technique. This study included consecutive 10 patients underwent salvage radiotherapy (RT) for biochemical relapse of prostate cancer after RP. Norma...

Seok Jin Kim ([email protected]) Chul Won Choi ([email protected]) Yeung-Chul Mun ([email protected]) Sung Yong Oh ([email protected]) Hye Jin Kang ([email protected]) Soon Il Lee ([email protected]) Jong-Ho Won ([email protected]) Min Kyoung Kim ([email protected]) Jung Hye Kwon ([email protected]) Jin Seok Kim ([email protected]) Jae-Yong Kwak ([email protected]) Jung Mi Kwon (aseve...

Acetyl-coenzyme A carboxylase (ACCase) occurs in at least two forms in rapeseed (Brassica napus): a homomeric (HO) and presumably cytosolic isozyme and a heteromeric, plastidial isozyme. We investigated whether the HO-ACCase of Arabidopsis can be targeted to plastids of B. napus seeds. A chloroplast transit peptide and the napin promoter were fused to the Arabidopsis ACC1 gene and transformed i...

BACKGROUND Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. METHODS We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-...