objective: the aim of present study was cloning and expression of phic31 integrase cdna in a bacterial expression vector. thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. materials and methods: in this experimental study, phic31 cdna was subcloned into a prokaryotic expression vector and transformed into e.coli bl21 (de3). recombinant phic31 in...

It is possible to use transgenic plants, as bioreactors, for the production of recombinant inexpensive chemicals and drug components. Transient gene expression is an appropriate alternative to stable transformation because it allows an inexpensive and rapid method for expression of recombinant proteins in plant tissues. In recent years, plant viral vectors have been increasingly developed as su...

automatic analysis of human facial expressions is one of the challenging problems in machine vision systems. it has many applications in human-computer interactions such as, social signal processing, social robots, deceit detection, interactive video and behavior monitoring. in this paper, we develop a new method for automatic facial expression recognition based on facial muscle anatomy and hum...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

background and objectives: hbha and mtb32c have been isolated from culture supernatants of mycobacterium tu- berculosis (m. tuberculosis) and mycobacterium bovis (m. bovis) and their immunogenicity previously studies have been confirmed. in this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (mt- b32c-hbha), in producing new chimeric protein under t...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

objective: induced pluripotent stem cells are generated from somatic cells by direct reprogramming. these reprogrammed pluripotent cells have different applications in biomedical fields such as regenerative medicine. although viral vectors are widely used for efficient reprogramming, they have limited applications in the clinic due to the risk for immunogenicity and insertional mutagenesis. acc...

for high-throughput production of recombinant protein in escherichia coli ( e. coli ), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and gc content specially at n-terminal region should be considered. in the current study, the effect of decreasing the percentage of gc nucleotides and opt...

objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...