An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange chromatography on DEAE cellulose. The acid phosphatase gave a single band on SDS-polyacrylamide slab gel electrophoresis. The molecular weight of the aci...

BACKGROUND Plant-based foods have been used in traditional health systems to treat diabetes mellitus. The successful prevention of the onset of diabetes consists in controlling postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in aggressive delay of carbohydrate digestion to absorbable monosaccharide. In this study, five plant-based foo...

There exist many methods for the application of the Gram stain to histological sections, and with all of them there is generally no difficulty in recognising Gram-positive organisms. However, with most of the well-recognised methods Gram-negative bacteria are difficult to distinguish from the background. Twort's method distinguishes Gram-negative bacteria from the background but is relatively t...

The pace of modern life dwellers in big cities worldwide increases the demand for fast food or ready-to-cook meals. Dal curry is a common and popular meal item Bangladesh, but its long cooking time needs to be reduced satisfy consumer’s needs. So, this study aimed at developing analyzing mixed dal powder. Samples were prepared with 100% lentil (control), 25% green gram 75% (S1), black (S2), 50%...

During the course of investigations on riboflavin 5’-phosphate and flavin adenine dinucleotide-hydrolyzing enzymes in green gram (Phaseolus radiatus), we observed that crude extracts possessed high phosphatase activity against a variety of phosphorylated compounds. Despite intensive studies on the animal phosphatases, plant phosphatases have received very little attention. Pfankuch (1) purified...

Background: In the present study, redundant blanket applications of chemical fertilizer application to soil set foot into aquatic environment and cause eutrophication. Besides it low nutrient efficiencies, organic matter, imbalanced fertilization response influence decrease crop yield. To solve this serious problem localized delivery multi nutrients loaded with electrospun nanofibre through see...

In recent years, green synthesis of nanoparticles has attracted a great attention because of medicine and biological applications. In this work, bismuth oxide nanoparticles (Bi2O3 NP) was prepared via green synthesis using mentha pulegium aqueous extract after 24 h at 90°C. The product was characterized by ultraviolet-visible (UV-VIS) spectrophotometer, Fourier transform infrared (FTIR), X-ray ...

Roselle (Hibiscus sabdariffa L.) is one of the most important medicinal crops in many parts of the world. In this study, the effects of microelements, antioxidants, and bioagents on Fusarium oxysporum, F. solani, and Macrophomina phaseolina, the causal pathogens of root rot and wilt diseases in roselle, were examined under field conditions. Preliminary studies were carried out in vitro in order...

Bacterial species capable of decolorizing textile and laboratory dyes were isolated from textile effluent treatment plant. Two bacterial strains (one Gram negative and another Gram positive bacterial strain) were screened for their ability to decolorize Red H5BL (reactive dye), Thymol blue (acid dye), Malachite green, Crystal violet (Triphenyl dye) and Congo red (azo dye). The highest decoloriz...

Abstract Background Studies of plant extract-mediated synthesis nanoparticles is extensively explored and studied in recent time due to eco-friendly, cost-effectiveness minimal use toxic chemicals for synthesis. In this study, the Ag–TiO 2 nanocomposites (NCs) was carried out using Origanum majorana leaf extract under ultrasound irradiation. plays an important role as reducing capping agent (NC...