Random amplified polymorphic DNA (RAPD) bands that distinguish Meloidogyne hapla and M. chitwoodi from each other, and from other root-knot nematode species, were identified using a series of random octamer primers. The species-specific amplified DNA fragments were cloned and sequenced, and then the sequences were used to design 20-mer primer pairs that specifically amplified a DNA fragment fro...

Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiol...

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeats (ISSRs) markers were performed to detect the genetic diversity among 6 new rice lines and 4 cultivars with different responses to drought tolerance and establish specific DNA markers associated with drought tolerance. Among 16 RAPD primers tested, only 5 produced bands polymorphic between lin...

to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

Genetic relationship among 17 citrus commercially important cultivars, including local and some hybrid varieties of Pakistan was evaluated by RAPD (Random Amplified Polymorphic DNA) markers. Fifteen decamer primers were selected out of 25 primers that produced 153 polymorphic bands with 13bands per primer on an average with a total of 188 bands. To analyze the genetic similarity or dissimilarit...

Frequency of somaclonal variation in embryogenic calli and plants of Olea europaea L cv.Kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic DNA (RAPD) analysis. Radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in OMc medium supplemented with IBA (5 mgL-1) and 2-ip (0.5 mgL-1). Somatic embryogenesis was induced in calli of radic...

background: as for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. specific random amplified polymorphic dna (rapd) markers of 490 bp, 720 bp and 460 bp using the primers tv-5, opa-6 and opa-11, respectively, were reported. this was the first description of possible ge­n...

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Developme...

Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability ...

Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic b...