BACKGROUND AND THE PURPOSE OF THE STUDY The binding ability of a drug to serum albumin has influence on the pharmacokinetics of a drug. In the present study, the mutual interaction of anticancer drug erlotinib hydrochloride with bovine serum albumin (BSA) using fluorescence and UV/vis spectroscopy was investigated. METHODS The BSA solution (0.1 mM) was prepared daily in Tris buffer (0.05 mol ...

We examined the role of antigenic charge on the deposition of immune complexes (IC) in murine gloerulia Acute serum sickens nephritis was induced by injecting chemically modified cationic bovine serum albumin (cationic BSA). Controls were immunized with unmodified native BSA (native BSA), All mice were given a primary intraperitoneal immunization with 500 pg of the respective antigens plus Freu...

Lactococcus lactis subsp lactis BSA (L. lactis BSA) was isolated from a commercial fermented product (BSA Food Ingredients, Montreal, Canada) containing mixed bacteria that are used as starter for food fermentation. In order to increase the bacteriocin production by L. lactis BSA, different fermentation conditions were conducted. They included different volumetric combinations of two culture me...

DST Unit of Nanoscience (DST UNS), Them of Chemistry, Indian Institute of Technolo E-mail: [email protected]; Fax: +91-44-22 Department of Chemistry, Institute of N University, Seoul 133-791, Korea † Electronic supplementary information ( of BSA and Au30@BSA at higher and lo Au–Lyz, BSA–Lyz adducts, XPS spect dependent MALDI-MS of AgQC@BSA–Lyz. ‡ Contributed equally. Cite this: RSC Adv., 2015,...

The sample preparation of samples containing bovine serum albumin (BSA), e.g., as used in transdermal Franz diffusion cell (FDC) solutions, was evaluated using an analytical quality-by-design (QbD) approach. Traditional precipitation of BSA by adding an equal volume of organic solvent, often successfully used with conventional HPLC-PDA, was found insufficiently robust when novel fused-core HPLC...

Hypothetically either decreased nitric oxide (NO) or increased O(2) could initiate 20-HETE-mediated vasoconstriction associated with hemoglobin-based blood substitutes (HBOC). To test this hypothesis, we infused Tm-Hb, an HBOC with low O(2) affinity, into isoflurane-anesthetized Wistar (W) and Sprague-Dawley (SD) rats after exchanging 20% of their blood with Ringer lactate. For comparison we in...

We examined the role of antigenic electrical charge as a determinant of glomerular immune complex localization in the rabbit. Serum sickness nephritis was induced in groups of New Zealand white rabbits by daily 25-mg intravenous injections of bovine serum albumin (BSA) chemically modified to be cationic (pI > 9.5) or more anionic (pI, 3.5-4.6); an additional group received unmodified native BSA...

The effect of a naphthalimide pharmacophore coupled with diverse substituents on the interaction between naphthalimide-polyamine conjugates 1-4 and bovine serum albumin (BSA) was studied by UV absorption, fluorescence and circular dichroism (CD) spectroscopy under physiological conditions (pH = 7.4). The observed spectral quenching of BSA by the compounds indicated that they could bind to BSA. ...

Silica gels capable of inducing defined conformational changes in proteins were synthesized by polymerization of either 3,3,3-trifluoropropylmethoxysilane (3F) or (3-heptafluoroisopropoxy)propalethoxysilane (7F) with tetraethylorthosilicate (TEOS). Bovine serum albumin (BSA) and fluorescein isothiocyanate conjugated BSA (FITC-BSA) were encapsulated in the gels. The hydrolyzed TEOS sol mixing wi...