Our recent demonstration that many eukaryotic mRNAs contain sequences complementary to rRNA led to the hypothesis that these sequences might mediate specific interactions between mRNAs and ribosomes and thereby affect translation. In the present experiments, the ability of complementary sequences to bind to rRNA was investigated by using photochemical cross-linking. RNA probes with perfect comp...

as new pathogenic strains are emerging and threatening aquaculture development, myxosporeans (myxozoa) are receiving much attention in recent years. myxosporean taxonomy is traditionally based on morphology of the myxospore stage. molecular data on indian myxosporeans are rare. in this report, the 18s rrna gene sequence of myxobolus cuttacki infecting gill lamellae of minor carp labeo bata (ham...

background: bovine theileriosis results from infection with obligate intracellular protozoa of the genus theileria . the phylogenetic relationships between two isolates of theileria annulata , and 36 theile­ria spp . , as well as 6 outgroup including babesia spp . and coccidian protozoa were analyzed using the 18s rrna gene sequence. methods: the target dna segment was amplified by pcr. the pcr...

cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. this study has aimed to investigate the hyper variable region of the 18s rrna gene in cryptosporidium for exact parasite identification . dna was extracted from 26 fecal samples from which initially cryptosporidium oocysts were identified by ziehl-neelsen acid-fast , auramine phen...

The quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) technique has been increasingly used in endocrine disrupting chemicals (EDCs) research. Usually, an appropriate endogenous control gene is critical for Q-RT-PCR to normalize the errors and sample-to-sample variations that occur in the course of tissue collection, RNA isolation, and RT-PCR. In this study, we cl...

The nucleotide sequence of the rDNA 18S region isolated from diploid and tetraploid species of the amphibian Odontophrynus americanus was determined and used to predict the secondary structure of the corresponding 18S rRNA molecules. Comparison of the primary and secondary structures for the 2n and 4n species confirmed that these species are very closely related. Only three nucleotide substitut...

The nucleotide sequence has been determined for a 664-base pair region of maize (Zea mays L.) mtDNA which contains the 3' end of the 18S rRNA gene, the 5S rRNA gene, and an intergenic region of 108 base pairs. Specific regions of the 18S rRNA gene show striking homology with the corresponding gene in Escherichia coli.

Analysis of processing, assembly, and function of higher eukaryotic ribosomal RNA (rRNA) has been hindered by the lack of an expression system that enables rRNA to be modified and then examined functionally. Given the potential usefulness of such a system, we have developed one for mammalian 18S rRNA. We inserted a sequence tag into expansion segment 3 of mouse 18S rRNA to monitor expression an...

The gene encoding the ribosomal protein S19 (RPS19) is frequently mutated in Diamond-Blackfan anemia (DBA), a congenital erythroblastopenia. The consequence of these mutations on the onset of the disease remains obscure. Here, we show that RPS19 plays an essential role in biogenesis of the 40S small ribosomal subunit in human cells. Knockdown of RPS19 expression by siRNAs impairs 18S rRNA synth...