background: the present study was carried out to investigate the accurate status of ovine theileria infection in sheep from ahvaz and surrounding region, a tropical area southwest iran. methods: a pcr-rflp method based on 18s ribosomal rna gene was designed which could detect and differentiate theileria and babesia spp. and also differentiate main theileria species in sheep at the same time. 11...

The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis-phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by ...

Quantitative RT-PCR analysis. The following primers were used to amplify β-casein cDNA and 18S rRNA sequences: forward primer of the β-casein gene 5'-GCT CAG GCT CAA ACC ATC TC-3' and reverse primer 5'-TGT GGA AGG AAG GGT GCT AC-3'; forward primer of the 18S rRNA gene 5’-TCGGAACTGAGGCCATGATT-3’ and reverse primer 5’CCTCCGACTTTCGTTCTTGATT-3’. Fragments of β-casein gene and 18S rRNA were amplifie...

Truncated mouse ribosomal DNA (rDNA) genes were stably incorporated into rat HTC-5 cells by DNA-mediated cell transfection techniques. The mouse rDNA genes were accurately transcribed in these rat cells indicating that there is no absolute species specificity of rDNA transcription between mouse and rat. No more than 170 nucleotides of the 5' nontranscribed spacer was required for the accurate i...

Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic...

The nucleotide sequence of the gene coding for the 18S ribosomal RNA of maize mitochondria has been determined and a model for the secondary structure is proposed. Dot matrix analysis has been used to compare the extent and distribution of sequence similarities of the entire maize mitochondrial 18S rRNA sequence with that of 15 other small subunit rRNA sequences. The mitochondrial gene shows gr...

Some generally accepted viewpoints on the phylogenetic relationships within the molluscan class Gastropoda are reassessed by comparing complete 18S rRNA sequences. Phylogenetic analyses were performed using the neighbor-joining and maximum parsimony methods. The previously suggested basal position of Archaeogastropoda, including Neritimorpha and Vetigastropoda, in the gastropod clade is confirm...

considering the importance of theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of t. equi among dromedaries in central iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. for this purpose, a total of 161 blood samples from camels were examined in terms of t. equ...

Velocity sedimentation studies of RNA of Sarcophaga bullata show that the major rRNA species have sedimentation values of 26S and 18S. Analysis of the rRNA under denaturing conditions indicates that there is a hidden break centrally located in the 26S rRNA species. Saturation hybridization studies using total genomic DNA and rRNA show that 0.08% of the nuclear DNA is occupied by rRNA coding seq...

One of the proteins identified as being involved in ribosome biogenesis by high-throughput studies, a putative P-loop-type kinase termed Fap7 (YDL166c), was shown to be required for the conversion of 20S pre-rRNA to 18S rRNA. However, the mechanism underlying this function has remained unclear. Here we demonstrate that Fap7 is strictly required for cleavage of the 20S pre-rRNA at site D in the ...