in vitro propagation of rose (rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including indian subcontinent. the development of new cultivars for commerce is faced with new challenges. in vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

rose is considered as one of the most popular and important ornamental plants in the world. during the last decade, tissue culture, micropropagation as well as genetic engineering have prevailed in rose production industry. gene transfer to embryogenic callus, derived from different types of explants is currently one of the most popular methods of improvement of rose cultivars, presented in par...

koelreuteria elegans, popularly known as “flame gold” is an ornamental tree. in vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified ms medium. the highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-d from leaf segments. calli transferred in 1.5 mg/l bap resulted in efficient shoot regeneration...

thyme (thymus vulgaris l.) is an aromatic and medicinal plant which is very important for the herbal industry. in this research, in vitro selection for nacl tolerance was investigated in this plant. hypocotyl and shoot explants (apical meristem and cotyledonary leaves) of sterilized seedlings were cultured in ms medium supplemented with different combinations of 2, 4-dichlorophenoxyacetic acid ...

achillea millefolium belonging to the astreaceae family is an endangered medicinal plant of jordan and of its neighboring countries. as an alternative to seed propagation, an efficient micropropagation of a. millefolium and its subsequent rooting were developed as an option for its in vitro conservation. a maximum of 5.9 shoots per microshoot were obtained on murashige and skoog agar medium sup...

Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...

somatic embryogenesis was initiated in c. sativus l. from shoot meristems on ls medium containing bap (2×10-5 m)+naa (2×10-5 m). various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. matured embryos coul...

saffron (crocus sativus l.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of cellulase, pectinase and hemicellulase and embedded in ca-alginate beads. they were cultured with or without nurse cells in ms medium supplemented with 2,4-d and 6-benzylaminopurine at 25°c. after several changes of medium, cell-clusters appeared on the surface of the ca-algi...