The utility of [(18)F]WC-4-116, a PET tracer for imaging caspase-3 activation, was evaluated in an animal model of myocardial apoptosis. [(18)F]WC-4-116 was injected into rats at 3 hours after a 30 min period of ischemia induced by temporary occlusion of the left anterior descending coronary artery in Sprague-Dawley rats. [(18)F]WC-4-116 uptake was quantified by 1) autoradiography, 2) microPET ...

In a prospective follow up of 116 high risk infants, a 24 hour behavioural chart on seven consecutive days was analysed at seven and 12 weeks of age. Of children who manifested atopic disease at 2 years, 44/116 (38%), had shown significantly more fussing during the seventh, and colic type cry during the twelfth week than those who remained healthy (72/116, 62%).

Dariush Ilghari, Kirsty L. Lightbody, Vaclav Veverka, Lorna C. Waters, Frederick W. Muskett, Philip S. Renshaw and Mark D. Carr Department of Biochemistry, University of Leicester, Henry Wellcome Building, Leicester, LE1 9HN, U.K. Running title: Solution structure of M. tuberculosis EsxG•EsxH complex * To whom correspondance should be addressed: Prof. Mark Carr, Tel. (+44) 116 229 7075; Fax (+4...

Objective: The anti-cancer effect and mechanism of N1,N3-bis(3-methoxysalicylidene) diethylenetriamine (Valdien) in HCT-116 cells were assessed in this study. Methods: MTT was used to measure cytotoxicity of the Valdien. The appearance of apoptotic HCT-116 cells was detected by flow cytometry analysis. Apoptosis proteins, caspase family and Bcl-2 family were viewed by Western blotting. The Wnt1...

BACKGROUND Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown. OBJECTIVE To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRN...

.............................................................................................................. 113 Introduction .......................................................................................................... 113 Study area, material and methods ........................................................................... 114 Results ........................................

APLF is a forkhead associated-containing protein with poly(ADP-ribose)-binding zinc finger (PBZ) domains, which undergoes ionizing radiation (IR)-induced and Ataxia-Telangiectasia Mutated (ATM)-dependent phosphorylation at serine-116 (Ser(116)). Here, we demonstrate that the phosphorylation of APLF at Ser(116) in human U2OS cells by ATM is dependent on poly(ADP-ribose) polymerase 3 (PARP3) leve...