In the present study, genetic diversity among 16 collections of Stevia rebaudiana Bertoni was assessed to compare the efficiency of two-marker systems over one marker using RAPD and ISSR markers. How well these two marker systems discriminated the Stevia collections was compared. In fact, all the Stevia accessions included in the present study have been growing under different altitudes, rangin...

Grapevine (Vitis vinifera L.) is one of important economic fruit crops found in Salahaldin province \ Iraq. To examine the Molecular characterization and genetic relationships among nine grape varieties by using random amplified polymorphic DNA (RAPD) marker. Fifteen RAPD primers produced band (71, 56%), while two yielded monomorphic bands only. The size fragment ranged between 250-2700 bp, wit...

DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...

Frequency of somaclonal variation in embryogenic calli and plants of Olea europaea L cv.Kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic DNA (RAPD) analysis. Radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in OMc medium supplemented with IBA (5 mgL-1) and 2-ip (0.5 mgL-1). Somatic embryogenesis was induced in calli of radic...

Identified germplasm is an important component for efficient and effective management of plant genetic resources. Traditionally, cultivars or species identification has relied on morphological characters like growth habit or floral morphology like flower colour and other characteristics of the plant. Studies were undertaken for identification and analysis of genetic variation within 34 rose cul...

background: garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. the analysis of genetic diversity plays an important role in breeding programs. the rapd technique could be very effective in detecting genetic variation in garlic.  objective: the objective of the present work was to detect molecular polymorphism among iranian garlic ...

Two atypical Paracoccidioides brasiliensis strains (yeast form at room temperature) have been isolated from chronically infected patients living in Brazil. Different random primers were used to characterize these isolates and compare them to typical strains. The RAPD patterns allowed the differentiation of all the selected isolates. Their genetic distance ranged from 5% to 80% of non-shared ban...

Inorganic arsenic is a known human carcinogen, yet its mechanism of action remains unclear. Our previous study showed that arsenite significantly induces oxidative DNA adducts and DNA-protein cross-links in several mammalian cell lines. In the present study, we used the random amplified polymorphic DNA (RAPD) assay to evaluate the possible target in the genomic DNA of human lymphoblastoid cells...

in order to identify the sex marker in mahisefied, rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south caspian sea. polymerase chain reaction random amplified polymorphic dna (pcr-rapd) was performed using 124 primer sets. all bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed usin...