نتایج جستجو برای: ژن rab27a

تعداد نتایج: 16136  

2011
Giulia Bolasco Dhani C. Tracey-White Tanya Tolmachova Andrew J. Thorley Teresa D. Tetley Miguel C. Seabra Alistair N. Hume

Rab27 small GTPases regulate secretion and movement of lysosome-related organelles such as T cell cytolytic granules and platelet-dense granules. Previous studies indicated that Rab27a and Rab27b are expressed in the murine lung suggesting that they regulate secretory processes in the lung. Consistent with those studies, we found that Rab27a and Rab27b are expressed in cell types that contain s...

2011
Alistair N Hume Miranda S Wilson Dmitry S Ushakov Michael A Ferenczi Miguel C Seabra

Organelle motility is an essential cellular function that is regulated by molecular motors, and their adaptors and activators. Here we established a new method that allows more direct investigation of the function of these peripheral membrane proteins in organelle motility than is possible by analysis of the organelle movement alone. This method uses multi-channel time-lapse microscopy to recor...

2011
Kouichi Mizuno José S. Ramalho Tetsuro Izumi

Exophilin8/MyRIP/Slac2-c is an effector protein of the small GTPase Rab27a and is specifically localized on retinal melanosomes and secretory granules. We investigated the role of exophilin8 in insulin granule trafficking. Exogenous expression of exophilin8 in pancreatic β cells or their cell line, MIN6, polarized (exophilin8-positive) insulin granules at the cell corners, where both cortical a...

Journal: :Blood 2012
Mathieu Kurowska Nicolas Goudin Nadine T Nehme Magali Court Jérôme Garin Alain Fischer Geneviève de Saint Basile Gaël Ménasché

Cytotoxic T lymphocytes kill target cells via the polarized secretion of cytotoxic granules at the immune synapse. The lytic granules are initially recruited around the polarized microtubule-organizing center. In a dynein-dependent transport process, the granules move along microtubules toward the microtubule-organizing center in the minus-end direction. Here, we found that a kinesin-1-dependen...

2013
Héctor Peinado Maša Alečković Simon Lavotshkin Irina Matei Bruno Costa-Silva Gema Moreno-Bueno Marta Hergueta-Redondo Caitlin Williams Guillermo García Ayuko Nitadori-Hoshino Caitlin Hoffman Karen Badal Benjamin A. Garcia Margaret K. Callahan Jianda Yuan Vilma R. Martins Johan Skog Rosandra N. Kaplan Mary S. Brady Jedd D. Wolchok Paul B. Chapman Yibin Kang Jacqueline Bromberg David Lyden

Tumor-derived exosomes are emerging mediators of tumorigenesis with tissue-specific addresses and messages. We explored the function of melanoma-derived exosomes in the formation of primary tumor and metastases in mouse and human subjects. Exosomes from highly metastatic melanoma increased the metastatic behavior of primary tumors by permanently “educating” bone marrow (BM) progenitors via the ...

2016
Jong il Park Ha Yeon Lee Ji Eun Lee Cheol hwan Myung Jae Sung Hwang

Melanosomes are lysosome-related organelles with specialized capabilities of melanin synthesis and movement mediated by the Rab27a-Melanophilin-MyosinVa protein complex. In this study, we found that 2-methyl-naphtho[1,2,3-de]quinolin-8-one (MNQO) induced melanosome aggregation around the nucleus in melan-a melanocytes and in melan-a melanocytes/SP-1 keratinocyte co-cultures without inducing tox...

Journal: :Arthritis and Rheumatism 2008
Rachelle Donn Stuart Ellison Rebecca Lamb Thomas Day Eileen Baildam Athimalaipet V Ramanan

OBJECTIVE To investigate whether single-nucleotide polymorphisms (SNPs) within the genes PRF1, GZMB, UNC13D, and Rab27a, which are involved in natural killer cell dysfunction and known to contribute to the risk of hemophagocytic lymphohistiocytosis (HLH), confer an increased risk of susceptibility to systemic-onset juvenile idiopathic arthritis (JIA). METHODS Four SNPs across the PRF1 gene lo...

2010
Karen Piper Hanley Tom Hearn Andrew Berry Melanie J Carvell Ann-Marie Patch Louise J Williams Sarah A Sugden David I Wilson Sian Ellard Neil A Hanley

Neurogenin 3 (NGN3) commits pancreatic progenitors to an islet cell fate. We have induced NGN3 expression and identified upregulation of the gene encoding the Ras-associated small molecular mass GTP-binding protein, RAB3B. RAB3B localised to the cytoplasm of human β-cells, both during the foetal period and post natally. Genes encoding alternative RAB3 proteins and RAB27A were unaltered by NGN3 ...

2012
Yu Liu Yijiang Zhou Keqing Zhu

Cancer cells invade by secreting enzymes that degrade the extracellular matrix and these are sequestered in lysosomal vesicles. In this study, the effects of the selective lysosome lysing drug GPN and the lysosome exocytosis inhibitor vacuolin-1 on lysosome exocytosis were studied to determine their effect on glioma cell migration and invasion. Both GPN and vacuolin-1 evidently inhibited migrat...

Journal: :Journal of immunology 2014
Katarzyna Franciszkiewicz Marie Boutet Ludiane Gauthier Isabelle Vergnon Kelly Peeters Olivier Duc Benjamin Besse Geneviève de Saint Basile Salem Chouaib Fathia Mami-Chouaib

The lytic function of CTL relies on the polarized release of cytotoxic granules (CG) at the immune synapse (IS) with target cells. CTL also contain CCL5 in cytoplasmic storage vesicles (CCL5V) distinct from CG, the role of which, in regulating T cell effector functions, is not understood. Using human CD8(+) T cells specific to a lung tumor-associated Ag, we show in this article that CTL release...

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