BACKGROUND In order to define the virulence factors between Chinese herb-resistant uropathogenic E. coli and susceptible strains, the UPEC isolates were classified into two groups according to its resistance to Chinese herbs. MATERIALS AND METHODS The susceptibility profile of strains was determined by disk diffusion method. PCR systems were used to detect genes encoding papC, Aer, hly and cn...

A total of 71 Aeromonas strains were isolated in the south of Jiangsu Province China in order to analyze the difference ofAeromonas spp. distribution between diseased fish and water environment. The sequence of 16S rDNA and gyrB demonstrated that the 71 Aeromonas isolates could be divided into 4 species, including A. veronii (55), A. hydrophila (11), A. salmonicida (3) and A. media (2). A. vero...

UNLABELLED The only tuberculosis (TB) vaccine in use today, bacillus Calmette-Guérin (BCG), provides insufficient protection and can cause adverse events in immunocompromised individuals, such as BCGosis in HIV(+) newborns. We previously reported improved preclinical efficacy and safety of the recombinant vaccine candidate BCG ΔureC::hly, which secretes the pore-forming listeriolysin O of Liste...

It has been shown that fluorescence resonance energy transfer (FRET)-based PCR, including the TaqMan assay and molecular beacons, has potential for rapid detection of pathogens. In these promising real-time detection assays a single internal oligonucleotide probe labeled on both the 5' (reporter) and 3' (quencher) ends is used for selective generation of fluorescence. In this paper, we describe...

We studied 100 well-characterized E. coli blood isolates from patients with urosepsis for their susceptibility to nalidixic acid, ampicillin and trimethoprim-sulfamethoxazole, according to prevalence of virulence factors, phylogenetic groups and subgroups, PAI II(J96)-like domains (determined by physical linkage of cnf1, hly and hra) and PAI I(CFT073)-like domains (determined by physical linkag...

Aeromonas hydrophila is a mesophilic motile aeromonad present ubiquitously and causes high number of fish mortalities particularly in India along with various intestinal extraintestinal diseases humans. In this study, total 88 samples their processed products were screened by PCR targeting species-specific 16S rRNA gene, the virulent genes such as aerA, hly ahh1. The findings study showed that ...