CPEB is a sequence-specific RNA-binding protein that regulates polyadenylation-induced translation. In Cpeb knockout mice, meiotic progression is disrupted at pachytene due to inhibited translation of synaptonemal complex protein mRNAs. To assess the function of CPEB after pachytene, we used the zona pellucida 3 (Zp3) promoter to generate transgenic mice expressing siRNA that induce the destruc...

Design and Subjects: We recruited 3450 individuals from 915 DZ twinning families (1693 mothers of twins) and 1512 controls of Caucasian origin. One mother of DZ twins was selected from 279 of the 915 families, and a DNA sample was screened for rare variants in GDF9 using denaturant HPLC. Variants were confirmed by DNA sequencing and genotyped in the entire sample by matrix-assisted laser desorp...

The size of the primordial follicle pool determines the reproductive potential of mammalian females, and establishment of the pool is highly dependent on specific genes expression. However, the molecular mechanisms by which the essential genes are regulated coordinately to ensure primordial follicle assembly remain a mystery. Here, we show that the small GTPase Rac1 plays an indispensable role ...

ObjectiveTo characterize and evaluate the variation in serum concentrations of oocyte-secreted growth differentiation factor 9 (GDF9) bone morphogenetic protein 15 (BMP15) throughout menstrual cycle women from young to advanced reproductive ages.DesignCross-sectional, observational, exploratory study.SettingMulticenter university-based clinical practices laboratories.Patient(s)Serum was collect...

Background: Oocyte in vitro maturation success rate is still low in compareision to oocyte in vivo maturation. Thus prediction and improvement of oocyte competency are two critical issues in assisted reproductive technology. New insights into oocyte-cumulus cell regulatory loop may assist our optimization of in vitro maturation technique. Maturation and developmental competency of cumulus oocyt...

The growth differentiation factor 9 (GDF9) gene plays a vital role in the and maturation of ovarian follicles laying hens. However, its messenger ribonucleic acid (mRNA) expression levels preovulatory indigenous chickens remain poorly understood. study aimed to identify association between egg weight quality traits, morphology, mRNA GDF9 pre-ovulatory South African Potchefstroom Koekoek chicken...

STUDY QUESTION Can high-resolution array comparative genomic hybridization (CGH) analysis of DNA samples from women with primary ovarian insufficiency (POI) improve the diagnosis of the condition and identify novel candidate genes for POI? SUMMARY ANSWER A mutation affecting the regulatory region of growth differentiation factor 9 (GDF9) was identified for the first time together with several...