نتایج جستجو برای: ژن اگزونوکلئاز1 exo1

تعداد نتایج: 16153  

2017
Anna-Lena Kolb Alasdair R. Gunn Nicholas D. Lakin

ADP-ribosyltransferases promote repair of DNA single strand breaks and disruption of this pathway by Poly(ADP-ribose) polymerase (PARP) inhibitors (PARPi) is toxic to cells with defects in homologous recombination (HR). Here, we show that this relationship is conserved in the simple eukaryote Dictyostelium and exploit this organism to define mechanisms that drive resistance of the HR-deficient ...

2014
Ellen Tsang Izumi Miyabe Ismail Iraqui Jiping Zheng Sarah A. E. Lambert Antony M. Carr

Genetic instability, a hallmark of cancer, can occur when the replication machinery encounters a barrier. The intra-S-phase checkpoint maintains stalled replication forks in a replication-competent configuration by phosphorylating replisome components and DNA repair proteins to prevent forks from catastrophically collapsing. Here, we report a novel function of the core Schizosaccharomyces pombe...

2013
Yi Zhou Tanya T. Paull

The resection of DNA double strand breaks (DSBs) initiates homologous recombination (HR) and is critical for genomic stability. Using direct measurement of resection in human cells and reconstituted assays of resection with purified proteins in vitro, we show that DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a classic non-homologous end joining (NHEJ) factor, antagonizes DSB resec...

2013
Sandra Muñoz-Galván Ana López-Saavedra Stephen P. Jackson Pablo Huertas Felipe Cortés-Ledesma Andrés Aguilera

While regulating the choice between homologous recombination and non-homologous end joining (NHEJ) as mechanisms of double-strand break (DSB) repair is exerted at several steps, the key step is DNA end resection, which in Saccharomyces cerevisiae is controlled by the MRX complex and the Sgs1 DNA helicase or the Sae2 and Exo1 nucleases. To assay the role of DNA resection in sister-chromatid reco...

Journal: :Nucleic acids research 2000
L S Symington L E Kang S Moreau

A plasmid gap repair assay was used to assess the role of three known nucleases, Exo1, Mre11 and Rad1, in the processing of DNA ends and resolution of recombination intermediates during double-strand gap repair. In this assay, alterations in end processing or branch migration are reflected by the frequency of co-conversion of a chromosomal marker 200 bp from the gap. Gap repair associated with ...

Journal: :Journal of Biological Chemistry 2019

2017
Dekang Liu Jane H. Frederiksen Sascha E. Liberti Anne Lützen Guido Keijzers Javier Pena-Diaz Lene Juel Rasmussen

DNA mismatch repair (MMR) is a highly-conserved DNA repair mechanism, whose primary role is to remove DNA replication errors preventing them from manifesting as mutations, thereby increasing the overall genome stability. Defects in MMR are associated with increased cancer risk in humans and other organisms. Here, we characterize the interaction between MMR and a proofreading-deficient allele of...

2016
Yuan Xue Marcus E Marvin Iglika G Ivanova David Lydall Edward J Louis Laura Maringele

Telomere attrition is linked to cancer, diabetes, cardiovascular disease and aging. This is because telomere losses trigger further genomic modifications, culminating with loss of cell function and malignant transformation. However, factors regulating the transition from cells with short telomeres, to cells with profoundly altered genomes, are little understood. Here, we use budding yeast engin...

Journal: :Genes & development 2008
Monica Segurado John F X Diffley

The DNA damage checkpoint plays a crucial role in maintaining functional DNA replication forks when cells are exposed to genotoxic agents. In budding yeast, the protein kinases Mec1 (ATR) and Rad53 (Chk2) are especially important in this process. How these kinases act to stabilize DNA replication forks is currently unknown but is likely to have important implications for understanding how genom...

Journal: :Genetics 2001
S Moreau E A Morgan L S Symington

MRE11 functions in several aspects of DNA metabolism, including meiotic recombination, double-strand break repair, and telomere maintenance. Although the purified protein exhibits 3' to 5' exonuclease and endonuclease activities in vitro, Mre11 is implicated in the 5' to 3' resection of duplex ends in vivo. The mre11-H125N mutation, which eliminates the nuclease activities of Mre11, causes an a...

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