A competitive enzyme-linked immunoassay has been devel oped to quantitate the M, 24,000 estrogen-regulated protein (p24) in human breast tumors and tumor cell lines. The concen tration of monoclonal antibody used to coat polystyrene microtiter assay plates was critical in optimizing assay sensitivity versus precision and demonstrated marked differences between plate types. Analysis of 114 human...

So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic cleavage of gag is strongly antigenic and is as conserved and immunogenic as p24. In some cases, antibodies to p17 are more prevalent than antibodies to p24 and the decline in...

and anterograde transport between the ER and Golgi (Strating & Martens, 2009), are required for Wg secretion by S2 cells. Similarly, knockdown by transgenic RNAi shows that p24 proteins are required for normal levels of Wg secretion in Drosophila wing imaginal discs (Buechling et al, 2011; Port et al, 2011). As with Evi, this requirement seems to be relatively specific, since general secretion ...

We wished to develop criteria for serological confirmation of human T-lymphotropic virus type I (HTLV-I) infection in healthy donors. Selected serum or plasma samples reactive by HTLV-I enzyme immunosorbent assay or gel-agglutination assays with at least one viral-specific band on Western immunoblot (WIB) were tested in six laboratories by four WIBs and four radioimmunoprecipitation assays (RIP...

HIV-1 isolation was attempted on 72 individuals, including persons with known HIV infection and five without proven HIV infection but with indeterminate Western blot patterns, as well as on low-risk HIV seronegative persons. The ability to detect HIV-1 from culture supernatant by p24 antigen capture assay was evaluated by segregating patients by absolute CD4+ cell counts, clinical stage of dise...

Three different groups of hemolysis mutants were produced by treatment of the M. pneumoniae FH-P24 strain with N-methyl-N-nitro-nitrosoguanidine. The first group of mutants, strains P24-L1, L2, and L11, showed wide and clear hemolytic zones, and attaching ability to erythrocytes and to hamster lung cells were the same as the properties of the parent strain and produced significant microscopic l...

BACKGROUND Bovine leukemia virus (BLV) is worldwide distributed and highly endemic in Argentina. Among the strategies to prevent BLV dissemination, a control plan based on the selective segregation of animals according to their proviral load (PVL) is promising for our dairy productive system. The objective of this work was to study the relationship between the blood PVL and the antibody level, ...

A taxonomic study was carried out on strain P24(T), which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium, enriched from a deep-seawater sample collected from the Indian Ocean. The isolate was Gram-negative, rod-shaped, motile by means of a polar flagellum, moderately halophilic and capable of reducing nitrate to nitrite. Growth was observed at salinities of 0-9 % and a...

A major factor in the design of highly sensitive ligandbinding assays is the need to accelerate the interaction between analyte and binding partner (or capture reagent). In early immunoassay technologies, overnight incubations with exogenously supplied binding partners at low temperatures (typically 2–8 °C) were common. More recently, however, assay results are frequently required, for both med...