The p25 triple gene block protein of Potato virus X (PVX) is multifunctional, participating in viral movement and acting as a suppressor of RNA silencing. The cell-to-cell movement of PVX is known to depend on the suppression function of p25. GFP-fused p25 accumulates in rod-like (RL) structures with intense fluorescence in cells. By monitoring the location of fluorescence at different times, w...

Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of ...

سابقه و هدف: سلامت خاک از مولفه ‏های اصلی در دستیابی به سامانه ‏‏های کشاورزی پایدار بوده که شدت تحت تاثیر عملیات زراعی مانند خاکورزی قرار می ‏گیرد. را توان با استفاده پارامترهای فیزیکی، شیمیایی بیولوژیکی قالب الگوریتم‏ های مشخص کمّی کرد. نتیجه، بررسی وضعیت کیفی باروری مدیریتی مختلف زمین جهت استقرار مناسب برای تولید بهینه نظام‌های امری ضروری می‏ باشد. چارچوب ارزیابی مدیریت SMAF 1 عنوان ابزاری قدر...

La uchuva es una de las frutas promisorias para la zona andina Colombia gracias a su alta demanda internacional. En Antioquia, siembra se concentra en el oriente, aunque otras subregiones como norte y suroeste presentan condiciones óptimas cultivo. Ya que enfermedades origen viral son uno los problemas emergentes este frutal, estudio evaluó infección virus RNA infectan cultivos Antioquia. Para ...

The novel viral vectors PVX-CP AltMV and PVXdt-CP AltMV are superexpressors of the capsid protein (CP). These viral vectors were constructed on the basis of the potato virus X (PVX) genome andAlternantheramosaic virus (AltMV) CP gene. The expression, based on the hybrid viral vectors, is genetically safe, since the systemic transport and formation of infective viral particles are blocked. CP Al...

We compared infection of Nicotiana benthamiana plants by the positive-sense RNA viruses Cucumber mosaic virus (CMV), Potato virus Y (PVY), and by a Potato virus X (PVX) vector, the latter either unaltered or expressing the CMV 2b protein or the PVY HCPro suppressors of silencing, at 25°C vs. 30°C, or at standard (~401 parts per million, ppm) vs. elevated (970 ppm) CO2 levels. We also assessed t...

Genes encoding plant nucleotide-binding leucine-rich repeat (NB-LRR) proteins confer dominant resistance to diverse pathogens. The wild-type potato NB-LRR protein Rx confers resistance against a single strain of potato virus X (PVX), whereas LRR mutants protect against both a second PVX strain and the distantly related poplar mosaic virus (PopMV). In one of the Rx mutants there was a cost to th...

Plant viral-based expression system offers an alternative tool for the expression of various target proteins via stable transformation or transient expression in plants. In transient expression strategy, generation of in vitro viral transcripts through complete linearization of the recombinant vector containing the cloned gene is crucial to ensure the success of gene expression in plants. Hence...

During infection, many RNA viruses produce characteristic inclusion bodies that contain both viral and host components. These structures were first described over a century ago and originally termed "X-bodies," as their function was not immediately appreciated. Whilst some inclusion bodies may represent cytopathic by-products of viral protein over-accumulation, X-bodies have emerged as virus "f...