Forty-nine isolates of Bradyrhizobium japonicum indigenous to a field where soybeans were grown for 45 years without inoculation were characterized by using four DNA hybridization probes from B. japonicum. nifDK-specific hybridization clearly divided the isolates into two divergent groups. Diversity in repeated-sequence (RS)-specific hybridization was observed; 44 isolates derived from 41 nodul...

Bradyrhizobium japonicum possesses three soluble c-type cytochromes, c550, c552, and c555. The genes for cytochromes c552 (cycB) and c555 (cycC) were characterized previously. Here we report the cloning, sequencing, and mutational analysis of the cytochrome c550 gene (cycA). A B. japonicum mutant with an insertion in cycA failed to synthesize a 12-kDa c-type cytochrome. This protein was detecta...

4 Department of Botany, Howard University, Washington, DC, USA A pronase-sensitive, 14 kDa component of bacteroids of Bradyrhizobium japonicum 1-1 IOARS was identified and characterized using monoclonal antibodies. This component was weakly synthesized or was missing in bacteroids of a unique Nod+ Fixmutant, ARS2525. Both the 14 kDa component and poly-/?-hydroxybutyrate (PHB) were located in th...

Schmidt 1973; Kapusta and Rouwenhorst 1973; Weaver and Frederick 1974). However, the numbers needed to overcome indigenous rhizobia are in many cases too excessive to be practical. In much of the soybean-growing area in the north central United States, an area encompassing many soil types, indigeneous strains of Bradyrhizobium japonicum serogroup 123 dominate nodulation of soybeans (Damirgi et ...

Rhizobia have a versatile catabolism that allows them to compete successfully with other microorganisms for nutrients in the soil and in the rhizosphere of their respective host plants. In this study, Bradyrhizobium japonicum USDA 110 was found to be able to utilize oxalate as the sole carbon source. A proteome analysis of cells grown in minimal medium containing arabinose suggested that oxalat...

an experiment was conducted at assossa agricultural research center (aarc) station during 2008 cropping season in order to study the effects of co-inoculation of bradyrhizobium japonicum (tal-378 and tal-379) and phosphate-solubilizing bacteria (psb) (pseudomonas spp.), and conventional farmers’ fertilizer level (combined and individual application of 46n kg ha-1 and 46 p2o5 kg ha-1) on nodulat...

The napEDABC genes of Bradyrhizobium japonicum encode the periplasmic nitrate reductase, an Mo-containing enzyme which catalyses the reduction of nitrate to nitrite when oxygen concentrations are limiting. In this bacterium, another set of genes, modABC, code for a high affinity ABC-type Mo transport system. A B. japonicum modA mutant has been obtained that is not capable of growing anaerobical...

Earthen pots experiments were conducted for the evaluation of nitrogen fixing potential of three indigenous Bradyrhizobium japonicum isolates and one commercial inoculum (Narmada biofertilizer) in three commonly grown soybean cultivars viz., JS-335, JS-9305 and JS-7322. Nitrogen fixation was assessed in terms of root nodules number, root and shoot length and their dry weight. Results indicate t...

Abstract The objective of this work was to evaluate the agronomic performance corn (Zea mays) grown single and intercropped with Urochloa ruziziensis in succession soybean (Glycine max) inoculated Bradyrhizobium japonicum, as well inoculation reinoculation Azospirillum brasilense, two contrasting soils. experiment carried out crop years, a randomized complete block design, 2x2x3 factorial arran...

Arachis hypogaea. L is a legume of economic importance, which is nodulated by Bradyrhizobium, a slow-growing bacteria. However there is no well characterization of this rhizobia in many areas of China. In the present study, cross-inoculation experiments were performed in cowpea and soybean. The isolated bacteria strains were characterized physiologyically, biochemically and id...