نتایج جستجو برای: ناقل rnai

تعداد نتایج: 13673  

Journal: :Current Opinion in Plant Biology 2021

Expression of insect-targeted RNA interference (RNAi) constructs in transgenic plants is a promising approach for agricultural pest control. Compared to conventional chemical insecticides, RNAi target specificity high and the potential negative environmental effects low. However, although numerous laboratory studies show insect growth inhibition by double stranded or artificial microRNA, few th...

2014
Koen W.R. van Cleef Joël T. van Mierlo Pascal Miesen Gijs J. Overheul Jelke J. Fros Susan Schuster Marco Marklewitz Gorben P. Pijlman Sandra Junglen Ronald P. van Rij

RNA interference (RNAi) is a crucial antiviral defense mechanism in insects, including the major mosquito species that transmit important human viruses. To counteract the potent antiviral RNAi pathway, insect viruses encode RNAi suppressors. However, whether mosquito-specific viruses suppress RNAi remains unclear. We therefore set out to study RNAi suppression by Culex Y virus (CYV), a mosquito...

RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...

ژورنال: :مجله دانشگاه علوم پزشکی اراک 0
حسین سرمد یان hosein sarmadian فرشیده دید گر farshideh didgar حمید ابطحی hamid abtahi

مقدمه: از آنجا که استافیلوکوک اورئوس یکی از مهم ترین عوامل بیماری زای انسان می باشد و با توجه به کلونیزاسیون این باکتری در بینی، پرسنل ناقل، این باکتری می تواند باعث افزایش میزان بروز عفونت های بیمارستانی شود. این مطالعه جهت مقایسه اثر دو رژیم ضد میکروبی (موپیروسین موضعی داخل بینی و سیپروفلوکساسین خوراکی) در درمان و عود ناقلین استافیلوکوک طراحی شده است. روش کار: این کار آزمایی بالینی به صورت سه...

Journal: :View 2021

The small interfering RNA (siRNA)-based therapeutics have raised great attention since the first interference (RNAi)-derived drug, patisiran, was approved by US Food and Drug Administration, which represented a landmark in field of gene therapy. Given properties disease-associated expression, RNAi machinery is regarded as an essential factor for preparing precise medicine. However, over past fe...

2012
Sherry C. Miller Keita Miyata Susan J. Brown Yoshinori Tomoyasu

The phenomenon of RNAi, in which the introduction of dsRNA into a cell triggers the destruction of the corresponding mRNA resulting in a gene silencing effect, is conserved across a wide array of plant and animal phyla. However, the mechanism by which the dsRNA enters a cell, allowing the RNAi effect to occur throughout a multicellular organism (systemic RNAi), has only been studied extensively...

Journal: :Philosophical Transactions of the Royal Society B: Biological Sciences 2009
Darren J. Obbard Karl H.J. Gordon Amy H. Buck Francis M. Jiggins

RNA interference (RNAi) is an important defence against viruses and transposable elements (TEs). RNAi not only protects against viruses by degrading viral RNA, but hosts and viruses can also use RNAi to manipulate each other's gene expression, and hosts can encode microRNAs that target viral sequences. In response, viruses have evolved a myriad of adaptations to suppress and evade RNAi. RNAi ca...

Journal: :Genetics 2013
Yanhui Hu Charles Roesel Ian Flockhart Lizabeth Perkins Norbert Perrimon Stephanie E Mohr

RNA interference (RNAi) is a widely adopted tool for loss-of-function studies but RNAi results only have biological relevance if the reagents are appropriately mapped to genes. Several groups have designed and generated RNAi reagent libraries for studies in cells or in vivo for Drosophila and other species. At first glance, matching RNAi reagents to genes appears to be a simple problem, as each...

Journal: :Microbiology 1999
U Binnie K Wong S McAteer M Masters

RNAI is a short RNA, 108 nt in length, which regulates the replication of the plasmid ColE1. RNAI turns over rapidly, enabling plasmid replication rate to respond quickly to changes in plasmid copy number. Because RNAI is produced in abundance, is easily extracted and turns over quickly, it has been used as a model for mRNA in studying RNA decay pathways. The enzymes polynucleotide phosphorylas...

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