Ref is an HNH superfamily endonuclease that only cleaves DNA to which RecA protein is bound. The enigmatic physiological function of this unusual enzyme is defined here. Lysogenization by bacteriophage P1 renders E. coli more sensitive to the DNA-damaging antibiotic ciprofloxacin, an example of a phenomenon termed phage-antibiotic synergy (PAS). The complementary effect of phage P1 is uniquely ...

The communication presents a numerical test of the three most popular methods used for direct inversion of the highly resolved IR and MW spectra of diatomic systems: (A) the numerical techniques such as those described by Coxon (J. A. Coxon and P. G. Hajigeorgiou, J. Mol. Spectrosc., 1991, 150, 1, ref. 1; J. A. Coxon, J. Mol. Spectrosc., 1992, 150, 274, ref. 2), Bernath (J. M. Campbell, M. Duli...

Pre-mRNA processing is mechanistically linked to transcription with RNA pol II serving as a platform to recruit RNA processing factors to nascent transcripts. The TREX complex member, REF/Aly, has been suggested to play roles in transcription and nuclear RNA stability in addition to its more broadly characterized role in mRNA export. We employed RNA-seq to identify a subset of transcripts with ...

OBJECTIVE Expression of adhesion molecules on endothelial cells and subsequent monocyte adhesion are initial events in the development of atherosclerosis. The purpose of this study was to investigate the role of apurinic/apyrmidinic endonuclease1/redox factor-1 (APE1/ref-1) in the interaction of monocytes with vascular endothelial cells. METHODS Human umbilical vein endothelial cells (HUVECs)...

Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a dual function protein; in addition to its DNA repair activity, it can stimulate DNA binding activity of numerous transcription factors as a reduction-oxidation (redox) factor. APE1/Ref-1 has been found to be a potent activator of wild-type p53 (wtp53) DNA binding in vitro and in vivo. Although p53 is mutated in most types of ...

Apurinic/apyrimidinic endonuclease 1 (Ape1/Ref-1) dysregulation has been identified in several human tumors and in patients with a variety of neurodegenerative diseases. However, the function of Ape1/Ref-1 is unclear. We show here that Ape1/Ref-1 increases the expression of glial cell-derived neurotropic factor (GDNF) receptor alpha1 (GFRalpha1), a key receptor for GDNF. Expression of Ape1/Ref-...

The mechanism of exclusion-sorption interaction of insulin-containing proteins with the column support in accordance with the chemical and three-dimensional structure in native and denaturating conditions was observed. The mechanisms of weak adsorption of linear proinsulin and fusion protein presenting in native, and dynamics of formation SDS-protein complex in denaturating conditions have been...