نتایج جستجو برای: روش lamp
تعداد نتایج: 379456 فیلتر نتایج به سال:
BACKGROUND & AIMS The pathogenic mechanism of pancreatitis is poorly understood. Recent evidence implicates defective autophagy in pancreatitis responses; however, the pathways mediating impaired autophagy in pancreas remain largely unknown. Here, we investigate the role of lysosome associated membrane proteins (LAMPs) in pancreatitis. METHODS We analyzed changes in LAMPs in experimental mode...
A genetic vaccine for West Nile virus (WN) has been synthesized with the WN premembrane-envelope (WN preM-E) gene sequences encoded as a chimera with the transmembrane and carboxyl terminal domains of the lysosome-associated membrane protein (LAMP). The LAMP sequences are used to direct the antigen protein to the major histocompatibility class II (MHC II) vesicular compartment of transfected pr...
شایع‌ترین ویروس تأثیرگذار بر کاهو در سراسر مزارع جهان، ویروس موزاییک کاهو (Lettuce mosaic virus; LMV) متعلق به خانواده Potyviridea و جنس Potyvirus است. روشهای متعددی شامل آزمون‌های سرولوژیکی و مولکولی جهت تشخیص این ویروس وجود دارند، اما این روش‌ها زمان‌بر بوده و نیازمند ابزارهای پیچیده‌ای هستند. هدف از این پژوهش، کاهش زمان موردنیاز جهت تشخیص LMV با استفاده از روش تکثیر هم‌دمای وابسته به حلقه (...
مقدمه و هدف: بیماری تیلریوز یکی از بیماریهای مهم و خطرناک مناطق گرمسیری و تحت گرمسیری می باشد که همه ساله در این مناطق مخصوصا ایران خسارت اقتصادی فراوانی را به صنعت دامپروری تحمیل می کند.هدف معرفی و کاربرد lamp در ردیابی انگل تیلریا ومقایسه آن با سایر روش های متداول آزمایشگاهی می باشد.lamp یک تکنیک پیشرفته مولکولی تکثیر dna است که به عنوان روشی سریع (حدود60-40دقیقه)، اختصاصی و ارزان جهت ردیابی ...
The loop-mediated isothermal amplification (LAMP) method, developed by our group for diagnosis of four human malaria parasites, was evaluated on a large scale at a remote clinic in Thailand where malaria is endemic. A total of 899 febrile patients were analyzed in this study. LAMP was first evaluated in 219 patients, and the result was compared to those of two histidine-rich protein (HRP)-2 rap...
A fluctuation-free mercury arc lamp for the pumping of a cw alexandrite laser is reported. Thermal insulation of a cathode of the lamp permits 4-kW fully vaporized direct-current operation. Fluctuations owing to the pool of liquid mercury in conventional lamp tubes is completely avoided. A 17-W, cw alexandrite laser pumped by the newly developed lamp is described.
A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of cytomegalovirus (CMV) was developed and evaluated. The LAMP assay specifically amplified only CMV DNA, and no cross-reactivity with the DNA of herpes simplex virus type 1, varicella-zoster virus, adenovirus, Aspergillus flavus, or Staphylococcus aureus was observed. The sequences of the LAMP assay-positive CMV prod...
The formation of brain circuits requires molecular recognition between functionally related neurons. We report the cloning of a molecule that participates in these interactions. The limbic system-associated membrane protein (LAMP) is an immunoglobulin (Ig) superfamily member with 3 Ig domains and a glycosyl-phosphatidylinositol anchor. In the developing forebrain, lamp is expressed mostly by ne...
Background and purpose: Exosome as drug delivery system is a novel and smart methodology enabling delivery of exosome cargo into specific tissue. This aim could be accessed by manipulation of exosome producer cells for expression of specific transmembrane-anchored ligand on exosomes surface. Accordingly, Lysosomal Associated Membrane Protein (LAMP) is one of the best choices for anchoring and c...
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