The impact of intracytoplasmic sperm injection (ICSI) on cryopreserved zygotes and embryos was evaluated by comparing embryo survival and implantation between embryos derived from ICSI and those derived from standard insemination procedures. The study included patients whose excess zygotes and embryos were cryopreserved between September 1993 and December 1994 and who subsequently underwent a f...

BACKGROUND To date, information on the pubertal development of adolescents born after ICSI is scarce, since the very first cohort is only now reaching young adulthood. In this study, pubertal development at the age of 14 was characterized in a longitudinally followed cohort of ICSI-conceived teenagers and compared with that of a spontaneously conceived (SC) control group. METHODS Pubertal dev...

Background: Intracytoplasmic Sperm Injection (ICSI) is one of the methods of assisted reproductive technology that aims to successfully treat male factor infertility in humans and domestic animals. The technique involves mechanically injecting a single sperm or sperm nucleus with the help of a micromanipulator into the cytoplasm of a mature metaphase II oocyte. Results: In domestic animals, ICS...

A couple with unexplained infertility was referred for routine IVF and ICSI treatment. Ovulation was induced by the GnRH analogue protocol combined with HMG and HCG. Preparation of denuded oocytes revealed a major disorder of the zona pellucida and abnormal oocytes. During preparation of ova for ICSI, 15 retrieved oocytes were denuded, 14 of which underwent ICSI treatment. Four of the oocytes c...

Intracytoplasmic sperm injection (ICSI) is not only used in human assisted reproduction (ART), but it is also widely employed for veterinary in vitro fertilization during rare species preservation, germ-line rescue of transgenic animals or for any veterinary assisted conception. Piezo-assisted ICSI, which was first described in 1995 [1], can be employed for assisted conception in animals in whi...

OBJECTIVE The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture. METHODS We retrospectively analyzed the outcomes of elective blastocyst culture performed between January 2011 and December 2014. RESULTS There were 2,003 cycles of conventional in vitro fertilization (IVF) and 336 cycles of intracytoplasmic sperm injection (...

The health economics of IVF and ICSI involve assessments of utilization, cost, cost-effectiveness and ability to pay. In 48 countries, utilization averaged 289 IVF/ICSI cycles per million of population per annum, ranging from two in Kazachstan, to 1657 in Israel. Higher national utilization of IVF/ICSI was associated with higher quality of health services, as indicated by lower infant mortality...

OBJECTIVE To determine if strict morphology correlates with outcome parameters in couples undergoing intracytoplasmic sperm injection (ICSI). DESIGN Retrospective review. SETTING Academic nonprofit IVF center. PATIENT(S) Couples undergoing IVF/ICSI. INTERVENTION(S) In vitro fertilization and ICSI. MAIN OUTCOME MEASURE(S) Samples were evaluated for total sperm count, motlity, progressi...

BACKGROUND Mouse oocytes can be used in conjunction with intracytoplasmic sperm injection (ICSI) as a technique to permit chromosomal analysis of human sperm. However, chromosomes derived both from the human sperm and the mouse oocyte appear simultaneously following ICSI. The present study focused on evaluating whether or not previously enucleated mouse oocytes are usable for the analysis of hu...

OBJECTIVE To evaluate the role of hyaluronic acid (HA) for sperm selection before intracytoplasmic sperm injection (ICSI). DESIGN Three prospective studies. SETTING Private assisted reproduction center in Italy. PATIENT(S) Study 1: 20 men. Study 2: 15 men. Study 3: 206 couples treated with ICSI on a limited number of oocytes per patient (1-3) in accordance with Italian IVF law. INTERVEN...