The importance of tracking outbreaks of foodborne illness and the emergence of new virulent subtypes of foodborne pathogens have created the need for rapid and reliable sub-typing methods for Escherichia coli O157:H7. Fourier transform infrared (FT-IR) spectroscopy coupled with multivariate statistical analyses was used for sub-typing 30 strains of E. coli O157:H7 that had previously been typed...

Instrumentation is described that has been constructed at the National Institute of Standards and Technology (NIST) for the measurement of regular re ̄ectance and transmittance over the 2±25 mm wavelength region. This includes both specialized accessories used with Fourier-transform infrared (FT-IR) spectrometers and laser-based systems for high optical density transmittance measurements. The FT...

Fifteen putative Streptomyces spp. isolated from soil were selected to be analysed using Fourier transform infrared (FT-IR) spectroscopy and 16S rRNA gene sequencing. Four colour groupings (groups 1–4) were obtained and described according to the colour of their substrate mycelia, aerial mycelia, spore mass and pigmentation. The dendrogram constructed using unsupervised cluster analysis of the ...

Fourier transform infrared (FT-IR) spectroscopy and chemometric techniques were used to discriminate five closely related Salmonella enterica serotype Enteritidis phage types, phage type 1 (PT1), PT1b, PT4b, PT6, and PT6a. Intact cells and outer membrane protein (OMP) extracts from bacterial cell membranes were subjected to FT-IR analysis in transmittance mode. Spectra were collected over a wav...

Fourier transform infrared (FT-IR) spectroscopy is a rapid, noninvasive technique with considerable potential for application in the food and related industries. We show here that this technique can be used directly on the surface of food to produce biochemically interpretable "fingerprints." Spoilage in meat is the result of decomposition and the formation of metabolites caused by the growth a...

Cancer is a disease that does great harms to the health of human beings. FT-IR spectroscopy could identify variability at the molecular level in biological specimens. It is a rapid and noninvasive method, which could be used intraoperatively to modify surgical procedures. The aim of this paper is to identify and separate cancer from colitis in endoscopic colon biopsies through the use of FT-IR ...

The analysis of volatile compounds for food products characterization is currently performed by gas chromatographic separation, often coupled with mass spectrometry. In our research the possibility of the application of the Fourier-transform infrared spectroscopy (FT-IR) technique to the cheese volatile fraction evaluation was investigated. An Evolved Gas Analysis (EGA) FT-IR prototype, develop...

This study aimed to evaluate metabolic fingerprinting by Fourier transform infrared (FT-IR) spectroscopy as a technique for investigating microbial communities and their activities in soil. FT-IR spectra from earthworm casts, and other ‘biosamples’, were compared using multivariate cluster analyses. The work formed part of a wider study to quantify the risk of horizontal gene flow and to assess...

Molecular typing by PCR fingerprinting using the single primer (GACA)4 was performed with 110 isolates of Cryptococcus neoformans. Seventy clinical isolates of C. neoformans var. neoformans from Germany (n = 52) and Africa (n = 18) were included. Of these, serotype A (C. neoformans var. grubii) accounted for 47 isolates, serotype D for 12 and serotype AD for 11. Fourier transform infrared (FT-I...

Yersinia enterocolitica and other Yersinia species, such as Y. pseudotuberculosis, Y. bercovieri, and Y. intermedia, were differentiated using Fourier transform infrared spectroscopy (FT-IR) combined with artificial neural network analysis. A set of well defined Yersinia strains from Switzerland and Germany was used to create a method for FT-IR-based differentiation of Yersinia isolates at the ...