The relative amount of H1 histone associated with isolated nucleosomes from calf thymus was determined as a function of the extent of DNA digestion by micrococcal nuclease. Generally the amount of H1 histone associated with mononucleosomes decreases with increasing digestion until 60% of the original H1 remains associated with DNA 150 base pirs or less in size. Coincidentally, H1 histone increa...

Rho family GTPases regulate many morphogenetic processes during vertebrate development including neural tube closure. Here we report a function for GEF-H1/Lfc/ArhGEF2, a RhoA-specific guanine nucleotide exchange factor that functions in neurulation in Xenopus embryos. Morpholino-mediated depletion of GEF-H1 resulted in severe neural tube defects, which were rescued by GEF-H1 RNA. Lineage tracin...

A chromatin associated protein kinase was used to add 3 moles of phosphate to seryl side chains of 1 mole of histone H1. The DNA binding properties of this in vitro phosphorylated H1 were compared with those of unmodified H1. Considerably more radioactive superhelical DNA was retained on nitrocellulose filters at 20mM-40mM NaCl by phosphorylated H1 than by unmodified H1. However, zone velocity ...

A Dictyostelium discoideum genomic library was screened using a degenerate oligodeoxyribonucleotide derived from the peptide, GPKAPT, obtained from the N terminus of purified histone H1. Two identical H1 clones were isolated. Comparative sequence data reveal a typical H1 three-domain structure with considerable homology to the globular domain of higher eukaryetic H1 histones, especially to plan...

Answer Consider the case where the initial value is H0 and M ′ = M1‖M2‖ . . . ‖MN . Let the notation H1(M1, I) denote the first iteration of the hash function using message block M1 and initial value I. H1(M1, H0) = EM1(H0)⊕H0 H1(M1, H0) = EM1(H0)⊕H0 by complementary Property of DES H1(M1, H0) = EM1(H0)⊕H0 = H1(M1,H0) A⊕B = A⊕B ∀A,B Thus H1(H0,M1) yields the same result as H1(H0,M1). In additio...

The reovirus outer capsid protein μ1 is responsible for cell membrane penetration during virus entry and contains determinants necessary for virus-induced apoptosis. Residues 582 to 611 of μ1 are necessary and sufficient for reovirus-induced apoptosis, and residues 594 and 595 independently regulate the efficiency of viral entry and reovirus-induced cell apoptosis, respectively. Two of three α-...

Upon infection of human T-cell leukemia virus type 1 (HTLV-1), the provirus is integrated into the host cell genome and subsequently packaged into chromatin that contains histone H1. Consequently, transcriptional activation of the virus requires overcoming the environment of chromatin and H1. To efficiently activate transcription, HTLV-1 requires the virally encoded protein Tax and cellular tra...

To obtain a contradiction, suppose that there is a cycle; this implies that so that there are q ≥ 2 different governments H1, . . . ,Hq such that φ (Hj) = Hj+1 for all 1 ≤ j < q, and φ (Hq) = H1. Without loss of generality, let H1 be the least competent of these governments. Take H = φ (H2) (if q > 2 then H = H3 and if q = 2 then H = H1). As φ is a political equilibrium, Vi (H) > Vi (H2) holds ...

Histone H1 is an abundant and essential component of chromatin whose precise role in regulating gene expression is poorly understood. Here, we report that a major target of H1-mediated regulation in embryonic stem (ES) cells is the X-linked Rhox homeobox gene cluster. To address the underlying mechanism, we examined the founding member of the Rhox gene cluster-Rhox5-and found that its distal pr...

The general form of non-entangling unitary maps for measurement schemes is determined. It is shown that any POVM admits a non-entangling measurement. We prove the following. Proposition 1 Let H1,H2 be complex separable Hilbert spaces, φ0 a unit vector in H2. Assume U : H1 ⊗ H2 → H1 ⊗ H2 is a unitary map such that for all φ ∈ H1, U(φ ⊗ φ0) = φ′ ⊗ φ′ for some unit vectors φ′ ∈ H1, φ′ ∈ H2. Then U...