2 Conversation with Jonathan F. P. Rose 3 Conversation with Sara Caples and Everardo Jefferson 4 Conversation with Sunil Bald 5 Conversation with Marion Weiss and Michael Manfredi 6 “Pedagogy and Place”; a discussion with Dean Robert A.M. Stern, Jimmy Stamp, and Alfie Koetter 8 Masters in Environmental Design Minor Architecture Destabilizing Major Narratives, a review 9 Spring 2015 Architectura...

The global regulatory locus sarA comprises a 375-bp open reading frame that is driven by three promoters, the proximal P1 and distal P3 and P2 promoters. We mutated the weaker P3 and P2 promoters to ascertain the effect of the change on SarA protein and target gene expression. Our results indicated that the solely active P1 promoter led to a lower SarA protein level, which has an effect on agr ...

MOTIVATION The recent discovery of tiny RNA molecules such as microRNAs and small interfering RNA are transforming the view of RNA as a simple information transfer molecule. Similar to proteins, the native three-dimensional structure of RNA determines its biological activity. Therefore, classifying the current structural space is paramount for functionally annotating RNA molecules. The increasi...

The expression of virulence determinants in Staphylococcus aureus is controlled by global regulatory loci (e.g., sar and agr). The sar locus is composed of three overlapping transcripts (sar P1, P3, and P2 transcripts from P1, P3, and P2 promoters, respectively), all encoding the 372-bp sarA gene. The level of SarA, the major regulatory protein, is partially controlled by the differential activ...