Magnesium aminoclays were synthesized and used to transform non-competent Escherichia coli JM109 using the exogenous plasmid pUC19. The structure determined for Mg is analogous 2:1 trioctahedral smectites such as talc, with an approximate composition R8Si8Mg6O16(OH)4, where R = CH2CH2NH2, morphologically arranged in layered sheets. employed a cationic vehicle that enabled passage of plasmids ac...

How repressors act at the molecular level to tmrn off genes is only now beginning to be worked out. Most vital to this understanding is whether the operator, defined genetically as the site for the action of a repressor, would turn out to be part of a DNA molecule, a region of a messenger RNA molecule, or even a protein. Now that two specific repressors (lactose and X) are available," 2 it is p...

over the years, the use of plastics has complicated the problem of disposal of solid wastes. one strategy to reduce plastic waste is the use of biodegradable plastics. a group of these plastics are polyhydroxyalkanoates (phas). to date more than 250 different microorganisms are known to synthesize and accumulate pha. most pseu-domonas strains are able to accumulate mcl-pha. in previous studies,...

The marine bacterium Vibrio fischeri serves as a model organism for a variety of natural phenomena, including symbiotic host colonization. The ease with which the V. fischeri genome can be manipulated contributes greatly to our ability to identify the factors involved in these phenomena. Here, we have adapted genetic tools for use in V. fischeri to promote our ability to conditionally control t...

Escherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in E. coli. Isopropyl-β-D-thiogalactoside (IPTG) is currently the most efficient molecular inducer for regulating this promoter's transcriptional activity. However, limitat...

Abstract The human prolactin antagonist ? 1-11 -G129R-hPRL is a 21.9 kDa recombinant protein with 188 amino acids that downregulates the proliferation of variety cells expressing receptors. Periplasmic expression proteins in E. coli has been considered an option for obtaining soluble and correctly folded protein, as alternative to cytoplasmic production. aim this work was, therefore, synthesize...