this article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (mtx) in plasma. samples were collocted from children receiving high-dose mtx at shafa hospital (ahvaz university of medical sciences, ahvaz, iran) at various times after the end of eachinfusion. plasma was deproteinized with trichloroacetic acid and the supernatant was i...

a rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. ofloxacin was used as an internal standard and separation carried on a novapak c18 column using a mobile phase of 0.01 m phosphate buffer (ph =2.6): methanol (82: 18 v/v). extraction of drug was performed from plasma by liquid-...

Analytical methods using high-performance liquid chromatography-diode array detection (HPLC-DAD) and high-performance liquid chromatography/mass spectrometry (HPLC/MS) were developed for the analysis of the following chloroacetanilide herbicide metabolites in water: alachlor ethanesulfonic acid (ESA); alachlor oxanilic acid; acetochlor ESA; acetochlor oxanilic acid; metolachlor ESA; and metolac...

Glutathione redox status is a commonly used oxidative stress biomarker. High-performance liquid chromatography-ultraviolet (HPLC-UV) and HPLC-electrochemical detection (HPLC-ECD) have been used to assess glutathione status but have potential limitations due to challenging sample preparation procedures or electrochemical signal degradation. Thus, this study aimed to validate an HPLC-ECD approach...

background and objectives: aflatoxins are mycotoxins produced by aspergillus flavus and aspergillus parasiticus that can contaminate human and animal foods, including corn, wheat, rice, peanuts, and many other crops resulting in the illness or death of human and animal consumers. the aim of this study was to detect aflatoxin b1, b2, g1, g2 and total aflatoxin  in kashkineh, a traditional irania...

background & aim: oleuropein has pharmacologic properties and is the main component of olive leaves which is considered to be the most pharmacologic compounds of olive (olea europaea l.). the aim of this work was to investigate the possible using of the extract of this plant in food industry (sponge cake) in order to take advantage of its medicinal properties.experimental: ethanolic extract...