Polymerase chain reaction (PCR) technique was applied to detect BHV-1 in bovine semen inoculated with BHV-1. The technique was found to be 10(6) times more sensitive than a non-isotopic dot-blot hybridization method in detecting viral genomic DNA. Of the three primer pairs used, the one chosen from glycoprotein gC appeared to be most sensitive as it could detect up to 0.01 TCID50 of BHV-1 in th...

آگاهی از میزان تاج‌پوشش درختان در مناطق شهری به‌علت تأثیرات آن کاهش آلودگی‌‌های هوا، آلودگی صوتی، جلوگیری باد، ذخیرة نزولات آسمانی، و کنترل رواناب‌های بسیار ضروری است. ازآن‌جا‌که استخراج با روش‌های دستی وقت‌گیر پرهزینه است، تکنیک‌های سنجش دور می‌توانند ابزار مناسبی برای تأمین این داده‌ها باشند. طبقه‌بندی شیء‌پایه، انتخاب پارامترهای بهینة قطعه‌بندی، به‌ویژه پارامتر مقیاس، اهمیت بسیاری دارد معمول...

Bovine respiratory disease (BRD) is the leading cause of morbidity and mortality in feedlot cattle. herpesvirus-1 (BHV-1) one main culprits BRD; however, research on BHV-1 hampered by lack suitable models for infection drug testing. In this study, we established a novel bovine tracheal organoid culture grown basement membrane extract type 2 (BME2) matrix compared it with air–liquid interface (A...

Glycoprotein B (gB) of bovine herpesvirus 1 (BHV-1) is essential for BHV-1 replication and is required for membrane fusion processes leading to virus penetration into the target cell and direct spreading of BHV-1 from infected to adjacent noninfected cells. Like many of the herpesvirus gB homologs, BHV-1 gB is proteolytically processed by furin, an endoproteinase localized in the trans-Golgi ne...

AIM Objective of the present study was to isolate bovine herpes virus Type 1 (BHV-1) from semen of infected bull and to adapt it onto embryonated eggs and Madin-Darby bovine kidney (MDBK) cell line. Further, the virus was identified by agar gel immunodiffusion (AGID) test. MATERIALS AND METHODS Semen samples were collected from five BHV-1 positive bulls previously confirmed for the presence o...

Continuously growing cell lines infected with the protozoan parasite Theileria annulata can readily be established by in vitro infection of leukocytes with the sporozoite stage of the parasite. The aim of the current study was to determine whether such transformed cell lines could be used as antigen presenting cells to analyse the antigenic specificity of bovine CD8 T cell responses to viral in...

Oncolytic viruses selectively replicate in cancer cells by exploiting biochemical differences between normal and tumor cells. Treatment with epigenetic modifiers such as 5-Azacytidine, a DNA methyltransferase inhibitor, increases the replication and cytotoxicity of oncolytic viruses in vivo and in vitro. The cotton rat is an attractive animal to study oncolytic viruses, as syngeneic models of b...