Domestic Balkan donkey (Equus asinus asinus) is a native donkey breed, primarily found in the northern and eastern regions of Serbia. The objective of the study was to analyze proteins of Domestic Balkan donkey milk during the lactation period (from the 45 to the 280 day) by applying lab-on-a-chip electrophoresis. The chip-based separations were performed on the Agilent 2100 Bioanalyzer in comb...

BACKGROUND Cow's milk allergy is one of the most common food allergies among younger children. We investigated IgE antibodies to milk, and IgE and IgG4 antibodies to casein, α-lactalbumin and β-lactoglobulin in cow's milk allergic (CMA) and non-allergic (non-CMA) children in order to study their clinical usefulness. METHODS Eighty-three children with suspected milk allergy (median age: 3.5 ye...

Abstract To investigate the influence of internal micellar structure on course enzymatic cross-linking especially in initial phase reaction, casein micelles isolated from raw milk via ultracentrifugation were incubated with microbial transglutaminase (mTG) comparison non-micellar sodium caseinate. Reactive lysine and glutamine residues identified using a label-free approach, based identificatio...

In this study, it was aimed to investigate α -S1-casein and β -lactoglobulin levels some biochemical mineral values in raw milk samples obtained from cows, buffaloes, sheep, goats raised Samsun. The study material consisted of 40 taken goats, sheep buffaloes after milking a farm Samsun province Bafra district. α-S1-casein β-lactoglobulin serum were determined using ELISA test kits. Calcium (Ca)...

The aims of this study were to estimate the effect cow breed on milk protein profile, and assess relationship between profile coagulation properties (MCP) predicted with mid-infrared spectroscopy (MIRS). dataset included 173,841 test-day observations 12,533 specialised (Holstein-Friesian [HF] Brown Swiss [BS]) dual-purpose cows (Simmental [SI] Alpine Grey) sampled in 488 multi-breed herds. Fixe...

In bovine species, β-casein (β-CN) is characterized by genetic polymorphism. The two most common protein variants are β-CN A² (the original one) and A¹, differing from A² for one amino acid substitution (Pro67 to His67). Several bioactive peptides affecting milk nutritional properties can originate from β-CN. Among them, β-casomorphin-7 (BCM7) ranging from amino acid 60 to 66 can be released mo...

The most polymorphic milk protein gene is β-casein; 13 protein variants are known in cattle. Milk protein genetic polymorphism has received considerable research interest in recent years because of possible associations between milk protein and economically important traits in livestock. The present study was undertaken to explore the genetic polymorphisms in exon 7 of β-casein and exon 4 of κ-...

Casein is the collective name for a family of milk proteins. In bovine milk, casein comprises four peptides: αS1, αS2, β, and κ, differing in their amino acid, phosphorus and carbohydrate content but similar in their amphiphilic character. Hydrophilic and hydrophobic regions of casein show block distribution in the protein chain. Casein peptides carry negative charge on their surface as a resul...