BACKGROUND Conventional corn dry-grind ethanol production process requires exogenous alpha and glucoamylases enzymes to breakdown starch into glucose, which is fermented to ethanol by yeast. This study evaluates the potential use of new genetically engineered corn and yeast, which can eliminate or minimize the use of these external enzymes, improve the economics and process efficiencies, and si...

Immobilized metal chelate affinity chromatography (IMAC) support was practiced for α -amylase immobilization. Poly(hydroxyethylmethacrylate-methacryloylamidotryptophan)-Ni [p(HEMA-MAT)-Ni ] nanospheres, average diameter 100 nm, were produced by surfactant free emulsion polymerization. Characterizations of p(HEMAMAT)-Ni nanospheres were carried out by Fourier transform infrared (FTIR) spectrosco...

Sea lettuce (Ulva pertusa) is a nuisance species of green algae that is found all over the world. East-Asian species of the marine gastropod, the sea hare Aplysia kurodai, shows a clear feeding preference for sea lettuce. Compared with cellulose, sea lettuce contains a higher amount of starch as a storage polysaccharide. However, the entire amylolytic system in the digestive fluid of A. kurodai...

it is now believed that the inhibition of carbohydrate hydrolyzing enzymes (ches) in the digestive tract can significantly prolong the overall carbohydrate digestion time and decrease the postprandial hyperglycemia after a meal. therefore, inhibitors of ches can be useful therapeutic approaches in the management of diabetes mellitus, especially in the type 2, and complications associated with t...

OBJECTIVES In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. METHODS Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymograp...

The current study deals with a digestive α-amylase in the larvae of Pieris brassicae L. through purification, enzymatic characterization, gene expression, and in vivo effect of a specific inhibitor, Acarbose. Although α-amylase activity was the highest in the whole gut homogenate of larvae but compartmentalization of amylolytic activity showed an equal activity in posterior midgut (PM) and ante...

This study aimed to evaluate in vitro free radical scavenging activity and anti-diabetic properties of extracts from the sprout Borassus fabellifier. Antioxidant was performed using DPPH, ABTS, FRAP, SO, NO inhibition methods. The enzymes (α-amylase α- Glucosidase inhibitory activities were investigated. Moreover, glucose adsorption diffusion also studied confirm potential effect B. fabellifier...

Introduction: Identification of serous acinar differentiation is the basic step for the diagnosis of acinic cell carcinoma (ACC). α-amylase was described to be specific for normal acinar cell but its affinity to malignant counterpart is being contentious. Recently discovered marker anoctamin (DOG1) specific for diagnosis of GISTs has been reported for its efficacy to stain the malignant acinar ...

Inhibition of α-amylase, enzyme that plays a role in digestion of starch and glycogen, is considered a strategy for the treatment of disorders in carbohydrate uptake, such as diabetes and obesity, as well as, dental caries and periodontal diseases. Plants are an important source of chemical constituents with potential for inhibition of α-amylase and can be used as therapeutic or functional food...