In this study, we developed a Phenotype MicroArray (PM) protocol to profile cellular phenotypes in Zymomonas mobilis, which included a standard set of nearly 2,000 assays for carbon, nitrogen, phosphorus and sulfur source utilization, nutrient stimulation, pH and osmotic stresses, and chemical sensitivities with 240 inhibitory chemicals. We observed two positive assays for C-source utilization ...

Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, and arabinose to ethanol. To enhance its genetic stability, several genomic DNA...

Bexon & Dawes (1970) reported that Zymomonas anaerobia NCIB 8227 grew in either amino acid or ammonium salt medium with glucose as the energy source, if supplemented with lipoic acid or biotin. Subsequently M. J. S. Dadds and P. A. Martin (unpublished observations) observed that calcium pantothenate supported growth of 2. anaerobia, but neither lipoic acid nor biotin did. The vitamin requiremen...

In the current investigation, woody stem of Moringa oleifera was processed by chipping and milling subsequently exposed to a combination pretreatments involving 3% v/v solution nitric acid autohydrolysis. The simultaneous saccharification fermentation (SSF) pretreated hydrolysate M. conducted using Zymomonas mobilis Kluyveromyces marxianus in occurrence commercial cellulase enzyme, Tween 80, so...