Catalysis is the most efficient strategy for the preparation of enantiopure products. Homogeneous and enzymatic catalyses are in many respects complementary in terms of substrate and reaction scope, operating conditions, enantioselection mechanism, etc. In terms of performance optimization, directed evolution methodologies outperform combinatorial ligand libraries. With the aim of combining the...

Understanding the early genesis of new enzymatic functions is one of the challenges in protein design, mechanistic enzymology, and molecular evolution. We have experimentally mimicked starting points in this process by introducing primitive iron and oxygen binding sites at various locations in thioredoxin, a small protein lacking metal centers, by using computational design. These rudimentary a...

Cobb 500 broilers were fed diets that were zinc deficient (35ppm), supplemented with 70ppm zinc sulfate, or 70ppm zinc sulfate + 35ppm organic zinc (MINTREXTM Zn or zincmethionine). Half the birds were given a coccidiosis vaccine (ADVENT® coccidiosis control) at day of age, and challenged with all three species on day 24. MINTREX Zn and zinc-methionine both significantly increased intestinal br...

Dopachrome tautomerase (DCT; EC 5.3.3.12) catalyses the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid in the mammalian eumelanogenic biosynthetic pathway. This enzyme, also named TRP2, belongs to a family of three metalloenzymes termed the tyrosinase-related proteins (TRPs). It is well known that tyrosinase has copper in its active site. However, the nature of the metal ...

In this work, an efficient synthesis of bionanohybrids as artificial metalloenzymes (Cu, Pd, Ag, Mn) based on the application enzyme a scaffold was described. Here we evaluated effect changing metal, pH medium, and amount in these metalloenzymes, where changes metal species size nanoparticles occur. These nanozymes were applied degradation hydrogen peroxide for their evaluation mimetics catalas...

The metal centres in metalloenzymes and molecular catalysts are responsible for the rearrangement of atoms electrons during complex chemical reactions, they enable selective pathways charge spin transfer, bond breaking/making formation new molecules. Mapping electronic structural changes at sites reactions gives a unique mechanistic insight that has been difficult to obtain date. development X-...

Enzymatic and homogeneous catalysis offer complementary means to produce enantiopure products. Incorporation of achiral, biotinylated aminodiphosphine-rhodium complexes in (strept)avidin affords enantioselective hydrogenation catalysts. A combined chemogenetic procedure allows the optimization of the activity and the selectivity of such artificial metalloenzymes: the reduction of acetamidoacryl...