1. The regional and subcellular distribution of the incorporation of sn-['4C]glycerol 3-phosphate into rat brain lipids in vitro was investigated and compared with the relative specific activity of various chemical and enzyme markers. The similarity between the subcellular distribution of this incorporation and of NADPH-cytochrome c reductase activity indicated that the synthesis of phosphatidi...

Superoxide dismutases (SODs) are the primary reactive oxygen species (ROS)-scavenging enzymes of the cell and catalyze the dismutation of superoxide radicals O2- to H2O2 and molecular oxygen (O2). Among the three forms of SOD identified, manganese-containing SOD (MnSOD, SOD2) is a homotetramer located wholly in the mitochondrial matrix. Because of the SOD2 strategic location, it represents the ...

After oral administration to humans, eltrombopag undergoes extensive cleavage of its hydrazine linkage to metabolites, which are exclusively eliminated in urine. In vitro, the cleavage pathway was not detected in systems using cytochrome P450 enzymes, renal or hepatic microsomes, or hepatocytes but was readily evident after anaerobic incubation with rodent cecal contents or human fecal homogena...

Cnidarians are the simplest metazoans to exhibit satiety after feeding. When hydra are fed to repletion, they close their mouths and cease to capture prey. As feeding stops, contractions of the tentacles and body column increase. Our earlier experiments showed that a gel chromatographic fraction of prey substances inhibits prey capture. We now present evidence that the same fraction reduces the...

To study enterotoxigenic Escherichia coli (ETEC) association to the gut of pigs, a simple and reproducible experimental model would be helpful. The aim of this experiment was to establish a model for studying the association of ETEC to the gut epithelium of pigs. Intestinal segments were prepared from 4 weaned pigs, which were tested susceptible to E. coli O149:F4 (homo- and heterozygotic; 2 pi...

The hydrogenases of the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii were activated under anaerobic conditions. Exposure o f whole cells and cell-free homogenates to air lead to a complete inactivation of the hydrogenases. The inactivation in whole cells of Scenedesmus is faster than the inactivation of the cell-free homogenate. Inactivation of the hy­ drogenases could be reve...