We describe a procedure for measuring total protein in urine. The method is simple, sensitive, and free of interference from drugs that are known to affect other commonly used methods. The CV for a 1.1 g/liter urine control in daily, routine use is 4.6%. The procedure involves adsorbing the protein onto cellulose powder, binding of Ponceau S dye by the protein, washing away excess dye, and elut...

A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified ...

An accelerated, direct immunofluorescent-antibody procedure was developed for the detection of Salmonella in food products. This method includes pre-enrichment and selective enrichment but eliminates many of the washing and smear treatments present in existing methods. Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, bi...

........................................................................................................ 8 POPULÄRVETENSKAPLIG SAMMANFATTNING ................................. 10 ABBREVIATIONS ........................................................................................... 13 FOREWORD .......................................................................................................

BACKGROUND AND OBJECTIVE Nodular thyroid disease is a common condition in our clinical practice, and fine needle aspiration biopsy (FNAB) is the diagnostic procedure of choice. Its main limitation is the number of non-diagnostic samples. Since the Bethesda criteria were implemented in 2007 (a consensus document on the morphologic criteria and diagnostic terminology for interpretation of thyroid...

The cycling parameters for BOX A1R PCR were modified and the primer-specific annealing temperature was optimized. Using Trout Lake Escherichia coli isolates, four different template preparation methods were compared. The cell washing, heat lysis, and DNA isolation methods all gave clear and bright band patterns with good reproducibility. The cell washing template preparation method for direct u...

In the preceding paper (1) a new method of preparing the normal pigment from urine has been described. It consisted essentially of extracting the pigment from acidified urine (pH about 4.0) with n-butyl alcohol, washing freely at different stages in the procedure with water, chloroform, benzol, amyl acetate, and amyl alcohol and finally obtaining the pigment product in a dry form by washing wit...

This paper describes an investigation of the phenomenon of mental pollution in a sample of 50 female victims of sexual assault. Feelings of mental pollution were assessed using an interview and a questionnaire. An experimental procedure was employed to determine if feelings of dirtiness and the urge to wash could be provoked by deliberate attention to the assault memory. Thirty (60%) of the 50 ...