The methods and results of a gas-liquid chromatographic analysis of bile acids in serum are presented. The analysis of bile acids in serum involves enzymatic hydrolysis (cholylglycine hydrolase), preparation of propionated methyl ester derivatives of bile acid and gas chromatographic procedure with 2. 5 % OV-1. Adequate separation of the individual bile acids, (cholic, chenodeoxycholic and deox...