An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric ac...

A major design goal of the Flask architecture is to separate the mechanisms of concurrency control and recovery management in database programming systems. This paper describes the DataSafe component of Flask, which is the second recovery mechanism to be implemented within the architecture and therefore provides a proof of concept. The DataSafe is closely based on the DB Cache mechanism, modifi...

The Flask architecture, which mainly contains object manager (OM) and security server (SS), is widely used to support flexible security policies in operating system. In nature, OM and SS should be isolated from each other to separate decision from enforcement. However, current implementation of Flask, such as SELinux and SEBSD, puts both OM and SS in the same address space. If one component is ...

The most preferred containers by Aedes aegypti were studied April and July (rainy and dry periods) in two Manaus neighbourhoods. In all, 2,700 premises and 13,912 containers were examined, most (87%) recorded outdoors. Out of the 13,100 inspected premises, only 1.6% showed to be positive for Aedes aegypti, summing up to 7,916 collected samples. Most frequently found containers outdoors in eithe...

A new strain ofActinomycete (S. albovinaceus) no.10/2 with a broad antifungal and antibacterial activitywas isolated from soil sample of Palestine.The medium composition for antibioticproduction fromstrain S.albovinaceus was optimized using shake-flask methodology.The parameters that resulted in maximum antibiotic production are: 1.25 % mannitol and 1 % malt extract were found to be best carbon...

The morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morpho...

Introduction Shake flask cultivation is nowadays a routine technique during process development for mammalian cell lines. During shaken culture, changes in agitation velocity, shaking diameter or shake flask size affect the hydrodynamics in the shake flask. This might be reflected in the growth of the cultured cells. Process parameters such as power input, mixing time, fluid velocity etc. have ...

A. 3-Amino-3-(hydroxymethyl)-1,5-dioxaspiro[5.5]undecane. A 1-L singlenecked, round-bottomed flask is equipped with an egg-shaped, Teflon®coated magnetic stirring bar (3.5 cm x 1.5 cm), capped with a rubber septum, flame-dried under vacuum, and cooled under an argon atmosphere (Note 1). After cooling to ambient temperature (21–23 °C), to the flask is added anhydrous N,N-dimethylformamide (DMF, ...