Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of ...

Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced alpha-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions betwee...

background escherichia coli is one of the most causative pathogen of urinary tract infection. urinary tract infections (utis) are the second most common cause of morbidity and remain a serious health concern among the clinicians. the severity of uti caused by uropathogenic e. coli (upec) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. little is kn...

The aim of this study was to investigate the characteristics of canine uropathogenic Escherichia coli (UPEC) and the interaction between canine UPEC and human bladder epithelial cells. Ten E. coli isolates collected from dogs with cystitis were analyzed for antimicrobial resistance patterns, the presence of virulence factors, and biofilm formation. The ability of these isolates to induce cytoto...

UPEC is responsible for morethan 90% of UTI. Conventional and molecular characterizations are essential for epidemiological surveillance as well as proper management of diseases. Virulence factors, antibiotic susceptibility, RAPD pattern, plasmid profile of the E. coli isolated from UTI cases were studied using standard methods. Antibiotic susceptibility pattern of 127 uropathogenic E. coli rev...

In this study, the association between virulence genotypes and phylogenetic groups among Escherichia (E.) coli isolates obtained from pet dogs and cats with cystitis was detected, and fingerprinting methods were used to explore the relationship among strains. Forty uropathogenic E. coli (UPEC) isolated from dogs (n = 30) and cats (n = 10) in Italy were analysed by polymerase chain reaction (PCR...

background: urinary tract infections are one of the most frequent health problems and uropathogenic escherichia coli is the major pathogen resulting utis. the severity of utis is caused by the expression of a large range of virulence factors.in this study, we evaluated the allelic frequency fimh gene, in upecs isolated from patients with utis. this study also aimed to determine the roles of c64...

In the remote Japanese community of Saku, a rural town in the Nagano Prefecture, a large proportion of outpatient urinary tract infections was caused by well-recognized globally dispersed clonal lineages of uropathogenic Escherichia coli (UPEC). However, most of these strains were drug susceptible, suggesting that factors other than selection pressure account for the clonal spread of drug-susce...

Serum resistance is an important virulence marker in uropathogenic E. coli (UPEC) but its pattern with respect to commensal gastrointestinal strains is less studied. We compared the serum resistance pattern of UPEC with commensal strains in stool samples from healthy individuals. A total of 40 isolates of UPEC (test) and intestinal E. coli (control) each was obtained from patients of all age gr...

The presence of adhesins is arguably an important determinant of pathogenicity for Uropathogenic Escherichia coli (UPEC). Antimicrobial susceptibilities were tested by agar dilution method, fifteen adhesin genes were detected by polymerase chain reaction, and multilocus sequence typing (MLST) was analyzed in 70 UPEC isolates and 41 commensal E. coli strains. Extended-spectrum β-lactamase (ESBL)...