PURPOSE Hydration and urinary alkalinization are essential for reducing renal dysfunction with high dose methotrexate (HDMTX). This report presents an analysis of institutional methods used to achieve adequate urinary alkalinization and output for patients receiving single agent HDMTX. Renal and metabolic parameters of tolerance were examined. METHODS Medical records of adult patients receivi...

We describe a novel infection-responsive coating for urinary catheters that provides a clear visual early warning of Proteus mirabilis infection and subsequent blockage. The crystalline biofilms of P. mirabilis can cause serious complications for patients undergoing long-term bladder catheterisation. Healthy urine is around pH 6, bacterial urease increases urine pH leading to the precipitation ...

Exposure to hyperoxia (500-600 torr) or low pH (4.5) for 72 h or NaHCO3 infusion for 48 h were used to create chronic respiratory (RA) or metabolic acidosis (MA) or metabolic alkalosis in freshwater rainbow trout. During alkalosis, urine pH increased, and [titratable acidity (TA) -[Formula: see text]] and net H+ excretion became negative (net base excretion) with unchanged [Formula: see text] e...

1. The renal clearance of salicylate has been measured in two groups of patients undergoing treatment by alkaline diuresis for salicylate poisoning. One group received mannitol and sodium lactate, the other acetazolamide and sodium bicarbonate. 2. The relationship between urine pH and salicylate clearance was found to be the same in both groups and similar to that shown at non-toxic concentrati...

Introduction Urine pH is critical for net acid and solute excretion, but the genetic factors that contribute to its regulation are incompletely understood. Methods We tested the association of single nucleotide polymorphisms (SNPs) from 16 genes related to ammonia (NH3) metabolism (15 biological candidates selected a priori, 1 selected from a previous genome-wide association study analysis) t...

In 1945, a method was described by Bonsnes and the writer (1) for the determination of urinary creatine which involved only the addition of picric acid to dilute urine solutions and heating in a boiling water bath. An 80 per cent conversion of creatine to creatinine was obtained. The method has now been modified to achieve 100 per cent conversion, on the basis of Clark and Thompson’s recent stu...

of a yellow Fe3tcitrate complex [calibration curve (A = 390 nm) was linear from 52.9 to 696.6 pmol/L, n = 8, r = 1.000; SD = 0.9; F = 263224.4; average relative error 0.3%]. Phosphate, which can interfere, is eliminated by precipitation. The usual procedui-e is as follows: To 4 mL of sample, add 0.1 mL of NH4OH (30%) and 0.9 mL of Mg2 (0.2 mol/L) and filter. Then adjust the pH to 2(0.1 mL of 10...

Dairy cattle barns are a major source of NH3 emissions to the atmosphere. Previous studies have shown that the bedding material used in the barn can influence the magnitude of NH3 emissions, but little is known about which bedding characteristics are important in this respect. The aims of this study were to assess, at a laboratory scale, the relative importance of the chemical [pH, cation excha...

To determine whether bucolome (5-n-butyl-1-cyclohexyl-2,4,6-trioxoperhydropyrimidine), a nonsteroidal anti-inflammatory agent, can reverse diuretic resistance of furosemide in patients with nephrotic syndrome, we examined the inhibitory effect of bucolome on the protein binding of furosemide in serum and urine. Bucolome significantly inhibited the protein binding of furosemide not only in serum...

IN THE DETERMINATION of enzymes in serum, blood or urine, the sample is added to a buffer designed to bring the pH after admixture to the peak of the enzyme activity curve. Body fluids themselves, however, are buffered and the pH of each fluid, even when fresh, can vary within certain limits, e.g., blood can vary from about pH 7 to 7.8 and urine from about pH 4.6 to 8. In. addition, samples of ...