bipolaris oryzae causes rice brown spot disease which reduces rice yield with substantial global impact. streptomycetes isolated from rice fields of guilan province, iran, showed antifungal activity against the tested pathogen. from five active streptomyces spp. isolates, isolate g showed high antagonistic activity. to determine its taxonomical identity, its colonies characterized morphological...

An in vitro experiment was conducted to evaluate the effect of methylcellulose on the attachment of major cellulolytic bacteria on rice straw and its digestibility. Rice straw was incubated with ruminal mixture with or without 0.1% methylcellulose (MC). The attachment of F. succinogenes, R. flavefaciens and R. albus populations on rice straw was measured using real-time PCR with specific primer...

Current genome walking methods are cumbersome to perform and can result in non-specific products. Here, we demonstrate the use of partially overlapping primer-based PCR (POP-PCR), a direct genome walking technique for the isolation of unknown flanking regions. This method exploits the partially overlapping characteristic at the 3' ends of a set of POP primers (walking primers), which guarantees...

The universal primer three-primer approach can dramatically reduce the cost when genotyping the microsatellites. One former research reported four universal primers that can be used in singleplex and multiplex genotyping. In this study, we proposed an alternative suite of universal primers with four dyes for genotyping 8-12 loci in one single run. This multiplex method was tested on Tetranychus...

In contrast with random-primed RACE (1) and RNA-PCR (2) which can be done to amplify respectively specific 5'or 3'-cDNA ends, random-PCR (rPCR) can be used to amplify the whole cDNA population, from 5' to 3' ends, derived from small amounts of RNA transcripts. This quick rPCR method allows construction of whole cDNA libraries from as little as one eukaryotic cell, 100 parasites or bacteria, or ...

We have developed a DNA-based assay to reliably detect brown rot and white rot fungi in wood at different stages of decay. DNA, isolated by a series of CTAB (cetyltrimethylammonium bromide) and organic extractions, was amplified by the PCR using published universal primers and basidiomycete-specific primers derived from ribosomal DNA sequences. We surveyed 14 species of wood-decaying basidiomyc...

Chloroplast microsatellites (cpSSRs) provide a powerful tool to study the genetic variation and evolution of plants. We have investigated the usefulness of 39 primer pairs tagging cpSSR loci on a set of eight different genera of Leguminosae (Papilionoideae subfamily) and five species belonging to the genus Phaseolus. Thirty-six 'universal' primer pairs were retrieved from the literature, one wa...